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山楂为蔷薇科植物山里红Crataegus pinnatifida Bge. var. major N. E. Br. 或山楂 Crataegus pinnati- fida Bge. 的干燥成熟果实,焦山楂为其炒制品[1]。现代药理研究证明,焦山楂抑菌作用强于生山楂,而某些特定菌群与消化功能密切相关[2]。而山楂炒焦后产生新的物质—类黑素,类黑素是在食品热处理过程中形成的。目前,类黑素的抗菌活性已得到证实。大多数类黑素对微生物作用的研究都是在特定的微生物生长培养基中进行的,这些研究表明类黑素可以刺激微生物生长[3],也可以抑制微生物生长[4-5]。肠道菌群与人体健康密切相关,药物和功能食品可能通过调节肠道微生物来改善胃肠功能,帮助消化[6-7]。双歧杆菌和大肠杆菌是典型的有益菌和有害菌,双歧杆菌常被加入酸奶饮品中帮助消化。乙酸是双歧杆菌的主要代谢物质,随着乙酸的增多,pH值降低从而抑制大肠杆菌的生长繁殖。本实验通过研究山楂,焦山楂以及焦山楂炒制过程中产生的类黑素对大肠杆菌、双歧杆菌以及其代谢物乙酸的影响,探究“山楂炒焦长于消食导滞”的作用机制。
Effects of hawthorn and melanoidins on the in-vitro growth of Bifidobacterium and E.coli
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摘要:
目的 考察山楂饮片及类黑素对双歧杆菌和大肠杆菌体外生长的影响。 方法 参照2015版《中国药典》方法炮制焦山楂,通过大孔吸附树脂提取工艺对焦山楂中的类黑素进行分离提纯,紫外分光光度法检测类黑素;气相色谱法检测生山楂、焦山楂和类黑素对双歧杆菌和大肠杆菌生长期、稳定期和衰亡期乙酸含量的影响。 结果 初期生山楂和焦山楂对细菌的影响大于类黑素,中后期类黑素通过改变乙酸的生成抑制大肠杆菌生长代谢,以及改变双歧杆菌生长代谢规律,促进乙酸生成,调节肠道菌群。 结论 生山楂、焦山楂和类黑素均通过促进肠道菌群生长代谢,促进消化。其中,焦山楂对肠道菌群效果较好。 Abstract:Objective Effect of hawthorn and melanoidins on the in-vitro growth of Bifidobacterium and E.coli. Methods According to methods of the Chinese pharmacopoeia (2015),the charred hawthorn was prepared. The melanoidins in charred hawthorn were separated and purified by the macroporous resin extraction process. Ultraviolet spectrophotometry was used to detect melanoidins. The gas chromatography was used to detect the effects of hawthorn, charred hawthorn and melanoidins on the content of the acetic acid in Bifidobacterium and E.coli during growth, stable and decay period. Results In the early stage, the effects of hawthorn and charred hawthorn on bacteria were greater than melanoidins. In the middle and late stage, melanoidins inhibited the growth and metabolism of E.coli by changing the generation of acetic acid, and contributed to that of Bifidobacterium and also promoted the generation of acetic acid and regulate the intestinal flora. Conclusion Hawthorn, charred hawthorn and melanoidins all promote digestion by promoting the growth and metabolism of intestinal flora. Among them, charred hawthorn has a better effect on intestinal flora. -
Key words:
- melanoidins /
- charred hawthorn /
- gas chromatography /
- Bifidobacterium /
- E.coli
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