一阶导数光谱法测定烧伤Ⅱ号酊中盐酸小檗碱的含量
Quantitative determination of berberine hydrochloride in shaoshang Ⅱ hao tincture by first derivative UV-spctrophotometry
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摘要: 本文采用一阶导数光谱法可不经分离直接测定烧伤Ⅱ号酊中小檗碱的含量。测定波长为361.3nm,平均回收率为99.97%,RSD为0.47%(n=5).此法具有简便、快速、准确等优点。Abstract: A first derivative UV-spectrophotometric method was successfully used without seperation for the determination of berberine in shaoshang Ⅱ hao tincture. The wavelength of determination was 361.3nm, the average recovery was 99.97%, RSD was 0.47% (n=5).The method is simple, rapid and accurate.
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