高效毛细管电泳法分离模拟混合的何首乌中7种蒽醌类成分的研究
Separation of mimic-mixed 7 kinds of anthraquinone derivatives in polygonum multiflorum thunb by high performance capillary electrophoresis
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摘要: 目的 研究模拟混合何首乌中7 种恩醌类成分的分离。方法 添加有机溶剂的胶囊电动毛细管色谱法。结果 大黄素苷、大黄素、芦荟大黄素、大黄酸、大黄素甲醚苷、大黄酚和大黄素甲醚在25mmol/L硼砂含25mmol/LSDS和10% 乙腈(pH9.2) 的缓冲溶液中16min 内得到完全分离。结论 添加有机溶剂的胶囊电动毛细管色谱法对恩醌类成分的分离具有明显的优越性。
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关键词:
- 胶囊电动毛细管色谱法 /
- 何首乌 /
- 分离
Abstract: OBJECTIVE To study the separation of mimic-mixed 7 kinds of antraquinone derivatives. METHODS Micellar electrokinetic capillary chromatography with additive of organic solvent was used. RESULTS Emodin,chrysophanl,physcion,rhein,emodin-8-o-β-D-glucopyranoside,aloe-emodin,and Physcion-8-o-β-glucopyranoside were separated successfully in 16 min in the buffer of 25 mmol/L H 3BO 3(pH9.2)containing 25mmol/L SDS and 10% acetonitrile. CONCLUSION Micellar electrokinetic capillary chromatography with additive of organic solvent has potential advantages on separation of anthraquinone derivatives. -
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