HPLC法测定氨糖美辛肠溶片中吲哚美辛的含量
Determination of indometacin in glucosamine indometacin enteric coated tablets by HPLC
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摘要: 目的 建立HPLC法测定氨糖美辛肠溶片中吲哚美辛的含量。方法 采用SPHERI-5Rp-18色谱柱,以甲醇-水-乙腈-磷酸(60:25:15:0.04)为流动相。结果 吲哚美辛在10~60μg/ml范围内浓度与峰面积有良好的线性关系(r=0.9999),平均回收率为99.56%,RSD为0.65%.结论 本法准确、快速、重现性好。Abstract: OBJECTIVE To establish a method for the determination the indometacin content in glucosamine indometacin enteric-coated tablets by HPLC.METHODS Using SPHERI-5 RP-18 column,with methanol-water-acetonitrile-phosphoric acid(60:25:15:0.04) as the mobile.RESULTS An excellent linear relationship was obtained between the peak area and the concentration with the range of 10~60μg/ml.The average recoverary was 99.56%,the RSD was 0.65%.CONCLUSION This method is accurate,quick and reproducible.
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Key words:
- indometacin /
- glucosamine indometacin /
- HPLC /
- determination
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