HPLC法测定诺氟沙星胶囊含量的改进
Improvement of determination of norfloxacin capsules by HPLC
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摘要: 目的 改进诺氟沙星胶囊含量HPLC测定方法。方法 采用反相高效液相色谱法。色谱柱:SpherisorbC18柱,流动相:磷酸-三乙胺缓冲液:水:乙腈 (60:30:10),流速:1.0ml·min-1,检测波长:278nm.结果 诺氟沙星的浓度在 4.8~96μg·ml-1范围内线性关系良好,回归方程:A=2.19× 104+7.40×104C(r=0.99998),加样回收率平均值为 98.5%.RSD=0.5%,n=5).结论 用本方法测定诺氟沙星胶囊的含量快速、重现性好、专属性强、结果准确可靠。Abstract: OBJECTIVE Norfloxacin capsules was determined by a improved HPLC method. METHODS The separation was performed on a spherisorb C18 column.The mobile phase was composed of phosphoric acid-triethylamin buf fer-water-acet onitrile(60:30:10).The eluate with a flow rate of 1.0ml·min-1 was detected at the wavelength of 278nm.RESULTS The calibration curves were good linear within the range of 4.8~96μg·ml-1(r=0.99998).The average recovery of norfloxacin was 98.5%,with RSD of 0.5%(n=5).CONCLUSION The improved method is rapid,good repeatabitity,accurate,reliable and suitable for determination of norfloxacin capsules.
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Key words:
- Norfloxacin capsules /
- HPLC /
- determination of content
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