电导法测定多硫酸肝素钠中硫酸基含量
Determination of sulfate groups in polysulfated heparin sodium with conductometry
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摘要: 目的: 建立一种测定多硫酸肝素钠中硫酸基含量的方法。 方法: 将多硫酸肝素钠通过H+型阳离子树脂,用氢氧化钠对其洗脱液进行滴定,通过电导率的变化确定滴定终点,并用氯代十六烷基吡啶对结果进行校正,测定其硫酸基含量。 结果: 硫酸基的平均加样回收率为100.18%,RSD为3.67%。 结论: 本法简便准确,重复性较好。Abstract: Objective: To establish a method for the determination of the contents of sulfate groups in polysulfated heparin sodium. Methods: The contents of sulfate groups were determined by converting the Na+ of polysulfated heparin sodium into H+ with cation exchange resin and titrating with sodium hydroxide. The titration end-point was ascertained by variance of conductivity and the results were rectified by cetylpyridinium chloride. Results: The average recovery was 100. 18% ,RSD = 3. 67% . Conclusion: This mthod was simple,reproducible and accurate.
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Key words:
- polysulfated heparin sodium /
- sulfate group /
- cetylpyridinium chloride /
- assay
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