高效液相色谱法测定复方补骨脂冲剂中补骨脂素和异补骨脂素的含量
Determinnation of psoralen and isopsoralen in Compound Buguzhi granule by HPLC
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摘要: 目的:建立复方补骨脂冲剂中补骨脂素和异补骨脂素的含量测定方法。方法:采用高效液相色谱法,Agilent C18柱,流动相为甲醇-水(50:50),检测波长为246 nm。结果:补骨脂素在1.88-30.10μg/mL之间呈良好的线性关系,平均回收率为98.56%,RSD为1.83%;异补骨脂素浓度在1.52-24.30μg/mL之间呈良好的线性关系,平均回收率为97.82%,RSD为2.01%。结论:该方法简便、可靠、准确,可用于复方补骨脂冲剂的质量控制。Abstract: Objective :To establish the determination method of the content of psoralen and isopsoralen in compound Buguzhi granule. Methods :A HPLC method was adopted.This analysis was carried on a column of Waters RP-C18 with a mobile phase of water-methanol at the ratio of 5050.The detection wavelength was 246nm. Results :Within the range of 1.88～30.10 μg/mL,psoralen assumed fine linear relation between sample size and response,the average recovery was 98.56%,and RSD was 1.83%;within the range of 1.52～24.30 μg/mL,isopsoralen assumed fine linear relation between sample size and response,the average recovery was 97.82%,and RSD was 2.01%. Conclusion :This Method is simple,reliable and accurate and can be used in the quality control of Compound Buguzhi granule.
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Key words:
- Compound Buguzhi granule /
- HPLC /
- psoralen /
- isopsoralen /
- content determination
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