RP-HPLC法测定重楼中四种甾体皂苷的含量
Simultaneous determination of four steroidal saponins compounds in Paris ployphylla by RP-HPLC method
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摘要: 目的:建立反相高效液相色谱法同时测定重楼中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ4种皂苷的含量测定方法,考察重楼中4种皂苷的含量。方法:采用色谱柱:Agilent SB C18柱(4.6 mm×150 mm,5μm);流动相:乙腈-水,梯度洗脱,流速0.8 mL/min;紫外检测器,检测波长:210 nm。结果:4种重楼皂苷的标准曲线线性良好(r>0.999 8,n=5),回收率98.12%～104.3%,最低检测限11.8～21.0 ng,最低定量限38.4～75.0 ng。不同产地的重楼中4种甾体皂苷的含量差异较大。Abstract: Objective :To establish a simultaneous determination method of steroidal saponin Ⅰ、Ⅱ、Ⅵ、Ⅶ in Paris ployphylla by RP-HPLC. Methods :The column was Agilent SB C18(4.6 mm×150 mm,5 μm).The mobile phase was acetonitrile-water with gradient elution at a flow rate of 0.8 mL/min.The UV detection was performed at 210 nm. Results :The calibration curves showed good linearity(r>0.999 8,n=5).The recoveries of the four steroidal saponins were from 98.12% to 104.3%.The limits of detection(LOD) ranged from 11.8 to 21.0 ng and limits of quantification(LOQ) ranged from 38.4 to 75.0 ng for all the reference standards,respectively.The results showed that the contents of the four steroidal saponins in Paris polyphylla very from different producing area. Conclusion :The method is accurate,convenient,and suitable for the quantitative analysis of four saponins from Paris ployphylla.
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Key words:
- Paris ployphylla /
- saponin /
- RP-HPLC /
- determination
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