分泌型磷脂酶A2在人皮肤黑素瘤细胞和组织中的表达和分布
Expression and distribution of secretory phospholipase A2 in human skin melanoma cells and tissues
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摘要: 目的:比较分泌型磷脂酶A2(sPLA2)在人恶性黑素瘤A375细胞、黑素瘤组织与健康人皮肤细胞、组织中的表达和分布。方法:分别采用ELISA法和Western Blot法检测细胞上清液和细胞内sPLA2的表达,免疫组化法考察人皮肤组织中sPLA2的表达和分布。结果:恶性黑素瘤A375细胞上清液的sPLA2浓度为(177.27±13.57)pg/mL,明显高于人表皮HaCaT细胞的(21.42±5.05)pg/mL(P<0.05)和真皮成纤维细胞的(2.75±1.23)pg/mL(P<0.05);其细胞内sPLA2/GAPDH的灰度比值为7.03±1.31,亦明显高于HaCaT细胞的1.45±0.37(P<0.05)和成纤维细胞的0.31±0.11(P<0.05);黑素瘤组织中呈现大量sPLA2阳性细胞和颗粒,而健康人表皮和真皮组织中则未见该阳性细胞和颗粒。结论:sPLA2在人恶性黑素瘤A375细胞和黑素瘤组织中较健康皮肤细胞和组织均有较高表达和分布,提示sPLA2有可能成为皮肤黑素瘤靶向给药的新靶点。Abstract: Objective :To investigate the expression and distribution of secretory phospholipase A2(sPLA2) in A375 human malignant melanoma cells and melanoma tissues compared with normal human skin cells and tissues. Methods :The expression of sPLA2 in the clear supernatant and intra-cellular of cells was detected by ELISA method and Western Blot method,respectively.Immunohistochemical method was used to investigate the expression and distribution of sPLA2 in the human skin tissues. Results :The levels of sPLA2 in the clear supernatant of A375 malignant melanoma cells was(177.27±13.57) pg/mL,which was significantly higher(P<0.05) than the group of human epidermal HaCaT cells(21.42±5.05) pg/ml and the group of dermal fibroblast cells(2.75±1.23) pg/mL,respectively.The ratio of sPLA2/GAPDH in the A375 cells was(7.03±1.31),which was also significantly higher(P<0.05) than the group of human epidermal HaCaT cells(1.45±0.37) and the group of dermal fibroblast cells(0.31±0.11),respectively.Many sPLA2 positive cells and granules were presented in the tumor tissues.In contrast,positive cells and granules above were unobserved in the normal human epidermal and dermal tissues. Conclusion :sPLA2 is expressed and distributed at higher levels in A375 human malignant melanoma cells and melanoma tissues compared with the normal skin cells and tissues,indicating the sPLA2 maybe a novel trigger for targeted drug delivery to skin melanoma.
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Key words:
- secretory phospholipase A2 /
- melanoma /
- expression /
- distribution
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