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内生真菌经过长期进化与宿主植物形成互利共生的关系,植物可以为内生真菌提供生长所需的营养物质,内生真菌可以促进植物生长、协助对抗干旱与虫害等胁迫、产生结构新颖丰富的次生代谢物以及诱导植物次生代谢物积累[1]。植物内生真菌作为来源于药用植物的一种新兴微生物资源,在医药、农业和食品工业领域都展现出越来越广泛的应用前景[2]。
金线莲Anectochilus roxburhii为兰科金线兰属(Anectochilus)多年生草本植物,又名金线兰、金耳环、鸟人参等,在民间有“药王”、“金草”等美称,具有清热凉血、除湿解毒等功效,主产于我国南部如福建、台湾、浙江等地,富含多糖、内酯苷和黄酮及苷类等成分,具有抗炎、提高免疫力、保肝、抗肿降糖等生物活性[3, 4]。目前,有关金线莲内生菌的研究多聚焦于植株体内内生菌生物多样性[5]、促进宿主生长[6],以及提高宿主抗病性[7]等研究。对于金线莲内生菌次生代谢产物的研究相对有限[8, 9],从金线莲内生真菌次生代谢产物中发现结构新颖、具有显著药效活性的先导化合物具有较大潜能,有待进一步挖掘。
本研究选取从金线莲中分离得到的一株内生真菌株Aspergillus sp. J218,采用大米固体发酵法进行发酵,并从发酵产物的乙酸乙酯萃取物中分离纯化、鉴定得到10个化合物(图1)。所鉴定成分多为二聚萘并吡喃酮类化合物,提示该株内生真菌含有丰富的二聚化合成酶,对其生物合成潜能有进一步挖掘的价值。
Secondary metabolites of endophytic fungus Aspergillus sp. J218 from Anectochilus roxburhii
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摘要:
目的 研究从金线莲Anectochilus roxburhii中分离的一株曲霉属内生真菌Aspergillus sp. J218的次生代谢产物。 方法 利用Sephadex LH-20凝胶、硅胶和高效液相制备等色谱法从J218固体发酵产物的乙酸乙酯部位分离化合物,采用波谱方法对化合物进行结构鉴定。 结果 从金线莲内生真菌Aspergillus sp. J218的次生代谢产物中共分离得到10个化合物,分别鉴定为kotanin( 1 )、flavasperone( 2 )、aurasperone B( 3 )、fonsecinone B( 4 )、fonsecinone D( 5 )、tensidol A( 6 )、fonsecinone A( 7 )、fonsecinone C( 8 )、aurasperone A( 9 )和fonsecinone F( 10 )。 结论 从金线莲内生真菌曲霉属Aspergillus sp J218次生代谢产物分离鉴定多为二聚萘并吡喃酮类化合物,提示该菌株含有丰富的二聚化合成酶,可为后续探索该菌株中二聚萘并吡喃酮类化合物的生物合成途径提供线索。 -
关键词:
- 曲霉属 /
- 金线莲 /
- 内生真菌 /
- 次生代谢产物 /
- 二聚萘并吡喃酮类化合物
Abstract:Objective To isolate the secondary metabolites of endophytic fungus Aspergillus sp. J218 from Anectochilus roxburhii. Methods Different chromatographic methods, including Sephadex LH-20 and silica gel chromatography as well as HPLC, were used to isolate compounds from the EtOAc fraction of the solid fermentation of J218, and their structures were identified by spectral methods. Results Ten compounds were isolated from the fermentation of J218 and their structures were individually identified as kotanin( 1 ), flavasperone( 2 ), aurasperone B( 3 ), fonsecinone B( 4 ), fonsecinone D( 5 ), ensidol A( 6 ), fonsecinone A( 7 ), fonsecinone C( 8 ), aurasperone A( 9 ), and fonsecinone F( 10 ). Conclusion Most compounds isolated from endophytic fungus Aspergillus sp. J218 in Anectochilus roxburhii were identified as dimeric naphthopyrones. These results suggest that this strain contains rich dimerization synthase, which could provide clues for the further exploration of the rational biosynthesis pathway of dimeric naphthopyrones in this strain. -
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