RP-HPLC法测定沙棘叶发酵前后4种黄酮苷类成分含量的变化

董睿方; 王政; 陈泠; 舒志恒; 韩婷; 秦路平

doi:10.3969/j.issn.1006-0111.2017.06.011

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RP-HPLC法测定沙棘叶发酵前后4种黄酮苷类成分含量的变化

董睿方, 王政, 陈泠, 舒志恒, 韩婷, 秦路平

文章导航 > 药学实践与服务 > 2017 > 35(6): 526-529

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引用本文:

董睿方, 王政, 陈泠, 舒志恒, 韩婷, 秦路平. RP-HPLC法测定沙棘叶发酵前后4种黄酮苷类成分含量的变化[J]. 药学实践与服务, 2017, 35(6): 526-529. doi: 10.3969/j.issn.1006-0111.2017.06.011

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Citation:

DONG Ruifang, WANG Zheng, CHEN Ling, SHU Zhiheng, HAN Ting, QIN Luping. Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2017, 35(6): 526-529. doi: 10.3969/j.issn.1006-0111.2017.06.011

RP-HPLC法测定沙棘叶发酵前后4种黄酮苷类成分含量的变化

doi: 10.3969/j.issn.1006-0111.2017.06.011

1.
上海师范大学生命与环境科学学院, 上海 200234;第二军医大学药学院生药学教研室, 上海 200433
2.
第二军医大学药学院生药学教研室, 上海 200433
3.
第二军医大学药学院生药学教研室, 上海 200433;宁夏医科大学药学院生药学教研室, 宁夏银川 750004

基金项目: 全军后勤科研计划重大专项（AWS14C001）

Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC

1.
College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
3.
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Department of Pharmacognosy, School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China

摘要: 目的建立反向高效液相色谱（RP-HPLC）法比较沙棘叶发酵前后芦丁、异槲皮苷、水仙苷、异鼠李素-3-O-葡萄糖苷含量的变化。方法沙棘叶及其发酵茶水提液浓缩干燥后用70%乙醇溶解，分析采用HPLC法，Extend-C₁₈色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.1%甲酸水溶液，流速1 ml/min，检测波长为356 nm，柱温30℃。结果沙棘叶中芦丁含量较高。经过发酵后，除异槲皮苷含量略有升高外，异鼠李素-3-O-葡萄糖苷含量变化不大，芦丁、水仙苷含量均有所下降。上述4种成分在一定范围内呈现良好的线性关系，相关系数r均大于0.9997；精密度、重复性和稳定性良好；加样回收率在96%~103%之间。结论沙棘叶发酵降低了芦丁和水仙苷含量，增加了异槲皮苷含量。该法简便、可靠，可为沙棘叶和沙棘叶发酵茶的质量评价提供科学依据。
- 反向高效液相色谱 /
- 沙棘叶 /
- 发酵 /
- 黄酮苷
Abstract: Objective To compare the changes of four flavonoid glycosides in the leaves of Hippophae rhamnoides L. before and after fermentation. Methods The water extract of Hippophae rhamnoides L. leaves and its fermented tea were concentrated and desiccated. The dry extracts were dissolved in 70% ethanol. The chromatographic separation was performed with RP-HPLC method on an Extend-C₁₈ column (4.6 mm×250 mm, 5 μm). Acetonitrile-0.1% formic acid was selected as mobile phase at the flow rate of 1.0 ml/min. The detection wavelength was 356 nm and the column temperature was 30℃. Results The rutin content was high in the leaves of Hippophae rhamnoides L. After fermentation, isoquercitrin content was increased, while the contents of rutin and narcissoside were reduced and isorhamnetin-3-O-glucoside stayed unchanged. There was a good linear relationship between the concentration and peak areas of the four compounds (r>0.9997). The average recoveries were between 96%-103%. Conclusion This established method is rapid and reliable, which can be used for the quality control of Hippophae rhamnoides L. leaves and its fermented tea.
- RP-HPLC /
- Hippophae rhamnoides L. leave /
- fermentation /
- flavonoid glycosides

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RP-HPLC法测定沙棘叶发酵前后4种黄酮苷类成分含量的变化

doi: 10.3969/j.issn.1006-0111.2017.06.011

1. 上海师范大学生命与环境科学学院, 上海 200234;第二军医大学药学院生药学教研室, 上海 200433

2. 第二军医大学药学院生药学教研室, 上海 200433

3. 第二军医大学药学院生药学教研室, 上海 200433;宁夏医科大学药学院生药学教研室, 宁夏银川 750004

基金项目: 全军后勤科研计划重大专项（AWS14C001）

收稿日期: 2016-05-30
修回日期: 2017-09-11

关键词:

反向高效液相色谱 /

沙棘叶 /

发酵 /

黄酮苷

摘要: 目的建立反向高效液相色谱（RP-HPLC）法比较沙棘叶发酵前后芦丁、异槲皮苷、水仙苷、异鼠李素-3-O-葡萄糖苷含量的变化。方法沙棘叶及其发酵茶水提液浓缩干燥后用70%乙醇溶解，分析采用HPLC法，Extend-C₁₈色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.1%甲酸水溶液，流速1 ml/min，检测波长为356 nm，柱温30℃。结果沙棘叶中芦丁含量较高。经过发酵后，除异槲皮苷含量略有升高外，异鼠李素-3-O-葡萄糖苷含量变化不大，芦丁、水仙苷含量均有所下降。上述4种成分在一定范围内呈现良好的线性关系，相关系数r均大于0.9997；精密度、重复性和稳定性良好；加样回收率在96%~103%之间。结论沙棘叶发酵降低了芦丁和水仙苷含量，增加了异槲皮苷含量。该法简便、可靠，可为沙棘叶和沙棘叶发酵茶的质量评价提供科学依据。

English Abstract

Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC

1. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

3. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China;Department of Pharmacognosy, School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China

Received Date: 2016-05-30
Rev Recd Date: 2017-09-11

Keywords:

Abstract: Objective To compare the changes of four flavonoid glycosides in the leaves of Hippophae rhamnoides L. before and after fermentation. Methods The water extract of Hippophae rhamnoides L. leaves and its fermented tea were concentrated and desiccated. The dry extracts were dissolved in 70% ethanol. The chromatographic separation was performed with RP-HPLC method on an Extend-C₁₈ column (4.6 mm×250 mm, 5 μm). Acetonitrile-0.1% formic acid was selected as mobile phase at the flow rate of 1.0 ml/min. The detection wavelength was 356 nm and the column temperature was 30℃. Results The rutin content was high in the leaves of Hippophae rhamnoides L. After fermentation, isoquercitrin content was increased, while the contents of rutin and narcissoside were reduced and isorhamnetin-3-O-glucoside stayed unchanged. There was a good linear relationship between the concentration and peak areas of the four compounds (r>0.9997). The average recoveries were between 96%-103%. Conclusion This established method is rapid and reliable, which can be used for the quality control of Hippophae rhamnoides L. leaves and its fermented tea.