HPLC法测定华海乙肝泡腾颗粒剂中柴胡皂苷C的含量
Determination of saikosaponin C in Huahai-yigan granules by HPLC
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摘要: 目的 为控制华海乙肝泡腾颗粒剂的质量建立测定柴胡皂苷C的方法。方法 采用高效液相色谱法,色谱条件为:ODS柱,以甲醇-水(61:39)为流动相,流这 1.0ml/min,检测波长203nm.结果 平均回收率99.3%,RSD=1.34%.结论 此法可作为控制华海乙肝泡腾颗粒剂质量的方法之一。Abstract: OBJECTIVE To determine saikosaponin C in Huahai-yigan granules. METKODS A chromatography method of HPLC for the determination wan eat8blished an ODS column with methanol-water(61:39) as the me bile phase at 1.0ml/min flow-rate, detection wavelength at 203 nm. RESULTS The average recovery was 99.3% and RSD was 1.34%. CONCLUSIONS This method is available fur quality control of Huahai-yigan granules.
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Key words:
- Huahai-yigan granules /
- SBE /
- saikosaponin C /
- HPLC
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