高效液相色谱法测定百年康口服液中橙皮苷的含量
Determination of hesperidin in Bainiankang oral liquid by HPLC
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摘要: 目的: 建立百年康口服液中橙皮苷的含量测定方法。 方法: 采用HPLC法, 色谱柱为YWG C18(150mm×4.6mm, 5μm), 流动相为乙腈-0.25%磷酸水(20∶80);检测波长为285nm, 流速为1.0mL/min。 结果: 橙皮苷在5~50mg/L浓度范围内呈良好的线性关系(r=0.9996), 平均回收率为100.92%, RSD为1.78%。 结论: 本法灵敏、准确、重现性好、精密度高、专属性强, 可用于百年康口服液中橙皮苷的含量测定和质量控制。Abstract: Objective: To establish the determination of hesperidin in Bainiankang oral liquid. Methods: The determination was car-ried out by HPLC with a YWG C18(150mm×4.6mm, 5μm)column, acetonitrile-0.25% water(20:80)as mobile phase, detection wavelength was at 285nm, flow rate was 1.0 mL/min. Results: Hesperidin showed a good linear relationship at the range of 5~50mg/L, r=0.999 6.The average recovery was 100.92% and RSD was 1.78%. Conclusion: The method is sensitive, accurate, re-producible, specific and can be used for quality control of Bainiankang oral liquid.
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Key words:
- Hesperidin /
- HPLC /
- Bainiankang Oral Liquid
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