1300大孔吸附树脂分离通窍鼻炎片中总黄酮总皂苷的工艺研究
Studies on separation of total flavonoid and saponin in Tongqiao Biyan tablet with 1300 macroporous resin
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摘要: 目的: 研究1300大孔吸附树脂分离通窍鼻炎片中总皂苷总黄酮的工艺条件与参数。 方法: 以总黄酮与总皂苷的洗脱量为指标,考察大孔吸附树脂对通窍鼻炎片中总皂苷总黄酮的吸附性能和洗脱参数。 结果: 以160 mL样品液(生药0.5 g/mL)上大孔吸附树脂柱(φ2.5 cm×H75 cm,干重45 g),用蒸馏水2 BV、30%乙醇3 BV、70%乙醇4 BV依次洗脱,总黄酮与总皂苷富集于30%乙醇和70%乙醇洗脱液部位。 结论: 该法简单可行,分离纯化效果好,能满足于大生产的要求。Abstract: Objective: To study the technological parameters of separating total flavornoid and saponin of Tongqiao Biyan tablet with macroporous resin 1300. Methods: With the elution quantity as index,the absorption characteristics and elution parameters were stud- ied. Results: 160 mL extractive of Tongqiao Biyan tablet(crude drugs 0.5g/mL)was added on a column of macroporous resin(φ2.5 cm×H75cm,dry weight 45g)and was washed with 2 BV of distilled water,3 BV of 30% ethanol and 4 BV of 70% ethanol,succes- sively.Total flavonoid and saponin were enriched in the 30% and 70% ethanol elution. Conclusion: This method is simple and feasi- ble with good effect of separateion,which can meet industrial requirements.
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Key words:
- macroporous resin /
- Tongqiao Biyan tablet /
- total flavonoid /
- total saponin
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