紫外分光光度法测定盐酸奈福泮-β-环糊精包合物的含量
Determination of nefopam hydrochloride(NFH) in nefopam NFH-β-cyclodextrin inclusion compound by UV spectrophotometry
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摘要: 目的: 建立测定盐酸奈福泮-β-环糊精包合物中盐酸奈福泮含量的方法。 方法: 采用紫外分光光度法测定含量,测定波长为267 nm。用乙酸乙酯洗脱未包封的盐酸奈福泮,测定被包封的盐酸奈福泮含量及包封率。 结果: 盐酸奈福泮检测浓度在0.01~0.4 mg/mL范围内线性关系良好(r=0.9995,n=9);日内RSD =0.17%(n=5),日间RSD=1.82%(n=5);平均加样回收率为100.39%(n=9);测得3批样品的包封率分别为73.28%、70.37%、74.97%。 结论: 该方法操作简便、结果准确,适用于盐酸奈福泮-β-环糊精包合物中盐酸奈福泮含量的测定。
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关键词:
- 紫外分光光度法 /
- 盐酸奈福-β-环糊精包合物 /
- 盐酸奈福泮 /
- 含量测定
Abstract: Objective: To establish a method for the content determination of NFH in NFH-13-cyclodextrin inclusion compound. Methods: The drug concentration in NFH-β-eyelodextrin inclusion compound was detected at the wavelength of 267 nm by UV spectro- photometry.The free NFH was eluated by eetoaeetate.The enveloped NFH and its entrapment efficiency were detected by UV spectro- photometry. Results: The linear range of NFH was O.01~0.4 mg/mL (r=0.999 5,n=9);the within day RSD and between-day were 0.17% and 1.82% (u=5),respectively.The average recovery was 100.39% (n=9).The entrapment efficiencies of 3 batches of samples were 73.28%,70.37%,74.97%. Conclusion:: The method is simple and reliable,which can be used for the content determi- nation of NFH in NFH-β-cyclodextrin inclusion compound.
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