摘要:
目的:建立同时测定淫羊藿药材中5种成分的HPLC方法。方法:采用D iamonsil-C18柱(4.6mm×250mm,5μm);流动相:乙腈和甲酸水(pH=3.0),梯度洗脱;流速:0.9 mL/m in;DAD检测,检测波长:270 nm。结果:淫羊藿药材中5个主要黄酮类成分朝藿定A、朝藿定C、淫羊藿苷、箭藿苷A、宝藿苷Ⅰ分别在0.780 0~83.20μg/mL、2.081~222.0μg/mL、3.288~350.7μg/mL、0.8311~88.65μg/mL、0.351 8~37.52μg/mL的浓度范围内呈良好的线性关系,最低检测限分别为0.50、0.75、0.87、0.32、0.12μg/mL。重复性、稳定性实验结果的RSD<3%;各成分平均回收率均小于107%,RSD<6%。结论:该方法快速简便、准确可靠,各主要化学成分均能达到基线分离,适用于淫羊藿药材的质量控制。
Abstract:
Objective : To establish a HPLC method for simultaneous determination of five flavone constituents in Epimedium Brevicornum. Methods :The determination was performed on Diamonsil-C18 column(4.6mm×250mm,5μm,Dikma).The mobile phase was consisted of acetonitrile-formic acid aqueous solution with a flow rate of 0.9 mL/min.The detection wavelength was set at 270 nm. Results :A method has been established for determination of epimedin A1,epimedin B,icariin,sagittatoside A,baohuoside I in one run by HPLC. Conclusion :The established method is simple,convenient,accurate and reliable,and suitable for the quality control of Epimedium Brevicornum.