用细胞膜色谱法和HPLC-TOF/MS研究附子中的有效成分
Study on effective components from roots of Aconitum carmichaeli through cell membrane chromatography and HPLC-TOF/MS method
-
摘要: 目的 通过细胞膜色谱技术和高效液相-飞行时间质谱(HPLC-TOF/MS)技术相结合,初步筛选和鉴定附子中的有效成分。 方法 初步筛选采用自制的大鼠心肌细胞膜色谱柱,流动相为10 mmol/L 磷酸盐缓冲溶液,流速为0.2 ml/min。色谱分离采用SHISEIDO CAPCELL PAK C18柱,流动相为0.1％甲酸水溶液和乙腈,梯度洗脱,流速为1.0 ml/min。质谱鉴定采用TOF/MS,正离子模式扫描。 结果 确定了附子中8个可能的有效成分。 结论 通过细胞膜色谱技术和HPLC-TOF/MS技术相结合,为从天然产物中快速寻找新的活性成分提供了途径。
-
关键词:
- 细胞膜色谱 /
- 高效液相-飞行时间质谱 /
- 附子
Abstract: Objective To screen the effective components from roots of Aconitum carmichaeli by a cell membrane chromatography (CMC) combining with a HPLC-TOF/MS method. Methods Screening was conducted on a rat cardiac muscle CMC column, and mobile phase was 10 mmol/L phosphate buffer with a flow rate of 0.2 ml/min. Separation was conducted on a SHISEIDO CAPCELL PAK C18 column. The mobile phase consisted of 0.1% formic acid and acetonitrile using gradient elution with a flow rate of 1.0 ml/min. Identification was conducted by using TOF/MS in positive ion mode. Results Eight potential effective constituents were determined. Conclusion The combination of CMC and HPLC-TOF/MS method had provided a new way to discover active components targeting membrane receptors from natural products.-
Key words:
- cell membrane chromatography /
- HPLC-TOF/MS /
- Aconitum carmichaeli
-
[1] He LC, Yang GD, Geng XD. Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface[J]. Chin Sci Bull, 1999, 44(9): 826. [2] He LC, Wang SC, Geng XD. Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics[J]. Chromatographia, 2001, 54(1): 71. [3] 袁秉祥,贺浪冲,杨广德.细胞膜生物亲和色谱药物筛选系统[J].中国药理通讯,2003,20(3):68. [4] 方艺霖,张艺卜,肖小河,等.细胞膜色谱技术用于中药活性成分筛选的研究进展[J],中草药,2008,39(7):1119. [5] 聂 红,孟兰贞.细胞膜色谱及其在中药活性成分研究中的应用[J].中华实用中西医杂志,2004, 4 (17):334. [6] 阎爱荣,张 宏.附子的药理研究[J].中国药物与临床,2008,8(9):745. [7] 张菊花,张晓艳.探析不同炮制方法对附子药理作用及毒性的影响[J].海峡药学,2008,20(10):89. [8] 徐叔云,卞如濂,陈 修.药理实验方法学[M].第2版.北京:人民卫生出版社,l994:522. [9] 贺浪冲.细胞膜色谱法[D].西安:西北大学,1998:27. [10] 李 翔.四逆汤化学成分和抗心肌缺血作用的研究[D].上海:第二军医大学,2008:91. [11] Hu R, Zhao J, Qi LW, et al. Structural characterization and identification of C(19)-and C(20)-diterpenoid alkaloids in roots of Aconitum carmichaeli by rapid-resolution liquid chromatography coupled with time-of-flight mass spectrometry[J]. Rapid Commun Mass Sp, 2009, 23(11): 1619. -
点击查看大图
计量
- 文章访问数: 2699
- HTML全文浏览量: 219
- PDF下载量: 423
- 被引次数: 0
下载:
