中药复方二神丸主要化学成分的高效液相-飞行时间质谱分析
Analysis on chemical constituents of ershen pill by HPLC-TOF/MS
-
摘要: 目的 通过高效液相-飞行时间质谱(HPLC-TOF/MS)联用技术,对中药复方二神丸中的主要化学成分进行有效的鉴定。 方法 色谱分离采用资生堂 Capcell Pak C18 反相柱(3.0 mm×100 mm, 3 μm),流动相组成分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.6 ml/min;质谱定性采用飞行时间质谱,正离子模式扫描。 结果 在优化的液质联用条件下,通过飞行时间质谱鉴定出二神丸中21个成分,其中15种成分来自补骨脂,6种成分来自肉豆蔻。 结论 通过RRLC-TOF/MS联用技术,为鉴定二神丸中的化学成分建立起了一种快速、高效的分析方法。
-
关键词:
- 二神丸 /
- 高效液相-飞行时间质谱 /
- 化学成分
Abstract: Objective To analyze chemical constituents of ershen pill by rapid-resolution(high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A shelloseido column(3.0 mm×100 mm,3 μm) was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition, 21 major constituents in ershen pill were identified by time of flight mass spectrometry, among which, 15 constituents were from Psoralea corylifolia L,and 6 constituents were from Myristica fragrans Houtt. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of ershen pill.-
Key words:
- ershen pill /
- HPLC-TOF/MS /
- chemica1 constituents
-
[1] 王 缙,付庆会. 宋明期间二神丸和五味子散两方的发展演变[J]. 四川中医,2010,28:115. [2] 唐昌娟, 施 贝, 修彦凤,等. 二神汤中补骨脂素_异补骨脂素的药动学研究[J]. 上海中医药杂志,2011,45:72. [3] 李 冀, 邹大威, 杜雅薇,等. 二神丸与四神丸对脾虚泄泻大鼠分泌型IgA白介素-2含量影响的配伍比较研究[J]. 中华中医药学刊,2007,25:2437. [4] 李 冀, 邹大威, 杜雅薇,等. 二神丸与四神丸对脾虚泄泻作用的配伍比较研究[J]. 辽宁中医杂志,2007,34:728. [5] Zhan ZJ, Fan CQ, Ding J, et al. Novel diterpenoids with potent inhibitory activity against endothelium cell HMEC and cytotoxic activities from a well-known TCM plant Daphne genkwa[J]. Bioorg Med Chem,2005,13:645. [6] Zhu Z, Zhang H, Zhao L, et al. Rapid separation and identification of phenolic and diterpenoid constituents from Radix Salvia miltiorrhizae by high-performance liquid chromatography diode-array detection, electrospray ionization time-of-flight mass spectrometry and electrospray ionization quadrupole ion trap mass spectrometry[J]. Rapid Commun Mass Spectrom,2007,21:1855. [7] Zhao L, Lou ZY, Zhu ZY, et al. Characterization of constituents in Stellera chamaejasme L. by rapid-resolution liquid chromatography-diode array detection and electrospray ionization time-of-flight mass spectrometry[J]. Biomed Chromatogr,2008,22:64. -
点击查看大图
计量
- 文章访问数: 3674
- HTML全文浏览量: 257
- PDF下载量: 156
- 被引次数: 0
下载:
