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肝细胞癌循环肿瘤细胞与肿瘤转移和预后关系的研究

王真,  徐增辉,  郑罗凝,  王颖,  孙保木,  金华君,  钱其军

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文章导航 > 药学实践与服务  > 2015 >  33(2): 159-162,192
王真, 徐增辉, 郑罗凝, 王颖, 孙保木, 金华君, 钱其军. 肝细胞癌循环肿瘤细胞与肿瘤转移和预后关系的研究[J]. 药学实践与服务, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
引用本文: 王真, 徐增辉, 郑罗凝, 王颖, 孙保木, 金华君, 钱其军. 肝细胞癌循环肿瘤细胞与肿瘤转移和预后关系的研究[J]. 药学实践与服务, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
shu
WANG Zhen, XU Zenghui, ZHENG Luoning, WANG Ying, SUN Baomu, JIN Huajun, QIAN Qijun. Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
Citation: WANG Zhen, XU Zenghui, ZHENG Luoning, WANG Ying, SUN Baomu, JIN Huajun, QIAN Qijun. Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
shu

肝细胞癌循环肿瘤细胞与肿瘤转移和预后关系的研究

doi: 10.3969/j.issn.1006-0111.2015.02.018
  • 1.

    东方肝胆外科医院病毒基因治疗实验室, 上海 200438;东方肝胆外科医院生物治疗科, 上海 200438

  • 2.

    东方肝胆外科医院病毒基因治疗实验室, 上海 200438

  • 3.

    浙江理工大学生命科学学院, 杭州 310018

  • 4.

    东方肝胆外科医院中西医结合科, 上海 200438

  • 5.

    东方肝胆外科医院病毒基因治疗实验室, 上海 200438;东方肝胆外科医院生物治疗科, 上海 200438;浙江理工大学生命科学学院, 杭州 310018

基金项目: 国家自然科学基金(No.81301307);国家传染病防治重大专项(No.2013ZX10002-010-007);上海细胞治疗工程技术研究中心科研基金(No.12DZ2251600);上海市卫生局科研基金(No.20114179)

Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma

  • 1.

    Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;Department of Biotherapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

  • 2.

    Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

  • 3.

    College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China

  • 4.

    Department of Integration of Traditional Chinese and Western medicine, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

  • 5.

    Laboratory of Gene and Viral Therapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;Department of Biotherapy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China;College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China

  • 摘要: 目的 研究肝细胞癌(hepatocellular carcinoma, HCC)患者外周血循环肿瘤细胞(circulating tumor cell, CTC)与肿瘤转移和预后的关系。 方法 选取35例肝细胞癌患者, 应用密度梯度离心法联合免疫磁珠阴性富集法富集CTC, 然后利用染色体荧光原位杂交和细胞免疫荧光技术进行CTC鉴定并计数, 同时记录临床相关特征, 对数据进行统计学分析。 结果 所有患者均检出CTC, 阳性率为100%, CTC个数均值为(4.1±2.5) 个。对患者进行分组, Ⅰ组的CTC个数<5, Ⅱ组的CTC数量≥5。Ⅰ组、Ⅱ组的CTC数量差异有统计学意义(P=0.001), 患者的性别、年龄与肿瘤转移无关(P=0.581、0.531), CTC的数量与转移及预后有关(P=0.024、0.01),Ⅰ组、Ⅱ组的转移和预后差异有统计学意义。 结论 HCC患者外周血CTC的数量与肿瘤转移及预后有关,CTC的数量≥5时,可能肿瘤更具转移倾向,预后更差,患者生存期更短。
    Abstract: Objective To study the relationship between circulating tumor cells(CTCs) in peripheral blood of the patients with hepatocellular carcinoma (HCC) and its metastasis and prognosis. Methods 35 patients with hepatocellular carcinoma were collected as the research objects.CTCs density were enriched through gradient centrifugation and negative-immunomagnetic methods, then the cells were detected by chromosome fluorescence in situ hybridization combined with immunofluorescence tests to identify CTCs. The clinical characters were recorded and the data were statistically analyzed. Results All the patients were detected CTC positive. The number of CTC was (4.1±2.5).The patients were divided into 2 groups.Group Ⅰ included patients whose CTCs were <5,and others were included in group Ⅱ.The difference between number of group I and group Ⅱ had significant significance(P=0.001); Metastasis had nothing to do with patients' sex and age(P=0.581,0.531); The number of CTCs was related to metastasis and prognosis(P=0.024,0.01),and there was significant statistic significance between group I and group Ⅱ. Conclusion The number of CTCs was related to tumor metastasis.The tumor may be more prone to occur metastasis and may had worse prognosis,and the patients may had shorter life time when the number of CTCs was≥5.
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    [2] Pantel K, Brakenhoff RH. Dissecting the metastatic cascade[J]. Nat Rev Cancer, 2004, 4(6): 448-456.
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    [9] Wu C, Hao H, Li L, et al. Preliminary investigation of the clinical significance of detecting circulating tumor cells enriched from lung cancer patients[J]. J Thorac Oncol, 2009, 4(1): 30-36.
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    [14] 曹宇勃,许崇安. 循环肿瘤细胞预测肝细胞癌患者临床预后价值的Meta分析[J]. 中国全科医学, 2014, 17(12): 1394-1399.
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  • 收稿日期:  2014-11-01
  • 修回日期:  2015-01-29

肝细胞癌循环肿瘤细胞与肿瘤转移和预后关系的研究

doi: 10.3969/j.issn.1006-0111.2015.02.018
  • 1. 东方肝胆外科医院病毒基因治疗实验室, 上海 200438;东方肝胆外科医院生物治疗科, 上海 200438
  • 2. 东方肝胆外科医院病毒基因治疗实验室, 上海 200438
  • 3. 浙江理工大学生命科学学院, 杭州 310018
  • 4. 东方肝胆外科医院中西医结合科, 上海 200438
  • 5. 东方肝胆外科医院病毒基因治疗实验室, 上海 200438;东方肝胆外科医院生物治疗科, 上海 200438;浙江理工大学生命科学学院, 杭州 310018
    • 基金项目:  国家自然科学基金(No.81301307);国家传染病防治重大专项(No.2013ZX10002-010-007);上海细胞治疗工程技术研究中心科研基金(No.12DZ2251600);上海市卫生局科研基金(No.20114179)
  • 收稿日期: 2014-11-01
  • 修回日期: 2015-01-29

摘要: 目的 研究肝细胞癌(hepatocellular carcinoma, HCC)患者外周血循环肿瘤细胞(circulating tumor cell, CTC)与肿瘤转移和预后的关系。 方法 选取35例肝细胞癌患者, 应用密度梯度离心法联合免疫磁珠阴性富集法富集CTC, 然后利用染色体荧光原位杂交和细胞免疫荧光技术进行CTC鉴定并计数, 同时记录临床相关特征, 对数据进行统计学分析。 结果 所有患者均检出CTC, 阳性率为100%, CTC个数均值为(4.1±2.5) 个。对患者进行分组, Ⅰ组的CTC个数<5, Ⅱ组的CTC数量≥5。Ⅰ组、Ⅱ组的CTC数量差异有统计学意义(P=0.001), 患者的性别、年龄与肿瘤转移无关(P=0.581、0.531), CTC的数量与转移及预后有关(P=0.024、0.01),Ⅰ组、Ⅱ组的转移和预后差异有统计学意义。 结论 HCC患者外周血CTC的数量与肿瘤转移及预后有关,CTC的数量≥5时,可能肿瘤更具转移倾向,预后更差,患者生存期更短。

English Abstract

王真, 徐增辉, 郑罗凝, 王颖, 孙保木, 金华君, 钱其军. 肝细胞癌循环肿瘤细胞与肿瘤转移和预后关系的研究[J]. 药学实践与服务, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
引用本文: 王真, 徐增辉, 郑罗凝, 王颖, 孙保木, 金华君, 钱其军. 肝细胞癌循环肿瘤细胞与肿瘤转移和预后关系的研究[J]. 药学实践与服务, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
shu
WANG Zhen, XU Zenghui, ZHENG Luoning, WANG Ying, SUN Baomu, JIN Huajun, QIAN Qijun. Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
Citation: WANG Zhen, XU Zenghui, ZHENG Luoning, WANG Ying, SUN Baomu, JIN Huajun, QIAN Qijun. Study on the relationship of circulating tumor cells and metastasis and prognosis of hepatocellular carcinoma[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 159-162,192. doi: 10.3969/j.issn.1006-0111.2015.02.018
shu

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