Inhibitory effect of myricetin on the metastasis of human renal cell carcinoma
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摘要: 目的 研究杨梅酮体内外对人肾癌ACHN细胞株转移的影响。 方法 体外实验应用不同浓度的杨梅酮（25、50、100 μmol/L）刺激ACHN细胞后，采用CCK-8实验、划痕实验和Transwell实验检测杨梅酮对于细胞活力、迁移和侵袭能力的影响。体内实验，首先通过尾静脉注射ACHN细胞建立肾癌肺转移模型，然后通过腹腔注射杨梅酮（15 mg/kg），6周后观察肾癌肺微转移灶评估杨梅酮对肾癌转移的影响。 结果 CCK-8实验显示，与对照组相比，当杨梅酮浓度达到50 μmol/L作用24 h后，能够显著降低细胞活性（P=0.019），并且随着药物浓度增加或作用时间延长抑制效应会更加显著。划痕实验和Transwell侵袭实验显示杨梅酮能显著抑制ACHN细胞的迁移和侵袭能力，并呈药物浓度依赖特性。体内实验也证实，与对照组相比，腹腔注射杨梅酮可显著减少肾癌肺微转移灶的数目。 结论 杨梅酮能够有效地抑制肾癌ACHN细胞的活力、迁移和侵袭能力，具有潜在的应用于肾癌治疗的价值。Abstract: Objective To evaluate the role of myricetin in the metastasis of human renal cancer ACHN cells both in vitro and in vivo. Methods With different concentration of myricetin (25, 50, 100 μmol/L) acting on ACHN cells, the cell viability, migration and invasion were respectively measured with CCK-8 assay, wound healing migration assay and Transwell invasion assay in vitro. A lung metastatic model for human renal cell carcinoma was developed by tail vein injection with ACHN cells. The effect of myricetin on the metastasis of renal cell carcinoma was explored by intraperitoneal injection of myricetin in vivo. After 6 weeks, the micro metastases of the lungs in nude mice was examined. Results Compared to the control group, CCK-8 assay showed that the cell viability was significantly lower in the myricetin group at the concentration of 50 μmol/L for 24 h (P=0.019). The inhibitory effect became more significant with the increase in drug concentration or action time. In addition, myricetin also inhibits the migration and invasion of ACHN cells in a dose dependent manner. Intraperitoneal injection of myricetin significantly reduced the number of the micro metastases of renal cell carcinoma in the lungs of nude mice. Conclusion Myricetin effectively inhibits the cell viability, migration and invasion of human renal cancer ACHN cells. It has the potential therapeutic value in the treatment of renal cell carcinoma.
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Key words:
- renal cell carcinoma /
- myricetin /
- migration /
- invasion
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