留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码
x 关闭 永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

珊瑚共附生黄柄曲霉次级代谢产物研究

王洪亮,  姜文丽,  李冉,  许聪聪,  张文

downloadPDF
文章导航 > 药学实践与服务  > 2019 >  37(2): 151-155
王洪亮, 姜文丽, 李冉, 许聪聪, 张文. 珊瑚共附生黄柄曲霉次级代谢产物研究[J]. 药学实践与服务, 2019, 37(2): 151-155. doi: 10.3969/j.issn.1006-0111.2019.02.010
引用本文: 王洪亮, 姜文丽, 李冉, 许聪聪, 张文. 珊瑚共附生黄柄曲霉次级代谢产物研究[J]. 药学实践与服务, 2019, 37(2): 151-155. doi: 10.3969/j.issn.1006-0111.2019.02.010
shu
WANG Hongliang, JIANG Wenli, LI Ran, XU Congcong, ZHANG Wen. Study on the secondary metabolites of Aspergillus flavipes isolated from coral[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 151-155. doi: 10.3969/j.issn.1006-0111.2019.02.010
Citation: WANG Hongliang, JIANG Wenli, LI Ran, XU Congcong, ZHANG Wen. Study on the secondary metabolites of Aspergillus flavipes isolated from coral[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 151-155. doi: 10.3969/j.issn.1006-0111.2019.02.010
shu

珊瑚共附生黄柄曲霉次级代谢产物研究

doi: 10.3969/j.issn.1006-0111.2019.02.010
  • 1.

    海军军医大学药学院 上海 200433;陆军军医大学士官学校, 河北 石家庄 050000

  • 2.

    海军军医大学药学院 上海 200433

  • 3.

    安徽中医药大学, 安徽 合肥 230000

基金项目: 国家自然科学基金（41576157）

Study on the secondary metabolites of Aspergillus flavipes isolated from coral

  • 1.

    Research Center for Marine Drugs, School of Pharmacy, Naval Medical University, Shanghai 200433, China;Sergeant School of Army Medical University, Shijiazhuang 050000, China

  • 2.

    Research Center for Marine Drugs, School of Pharmacy, Naval Medical University, Shanghai 200433, China

  • 3.

    Anhui University of Chinese Medicine, Hefei 230038, China

  • 摘要: 目的 研究东沙短足软珊瑚（Cladiella sp.）来源的黄柄曲霉菌（Aspergillus flavipes）中的活性次级代谢产物。 方法 采用硅胶柱层析、凝胶柱层析、制备HPLC等分离手段对真菌发酵液乙酸乙酯提取物进行分离，运用现代波谱技术结合文献报道数据，对化合物的结构进行鉴定；采用核因子κB受体活化因子配基（RANKL）诱导小鼠骨髓单核巨噬细胞（bone marrow macrophage cells，BMMs）分化为成熟的破骨细胞，经抗酒石酸酸性磷酸酶（TRAP）特异性染色，对化合物抑制破骨细胞分化活性进行研究。 结果 从该株真菌中分离得到4个细胞松弛素类化合物，其结构鉴定为trichalasins H，aspergilluchalasin，aspochalasin I和aspochalasin D。体外活性测试结果显示，化合物3和4可不同程度抑制BMMs向破骨细胞分化。 结论 对化合物3和4抑制破骨细胞分化活性的报告是本文首次报道，对新型抗骨质疏松活性物质研究具有科学价值。
    Abstract: Objective To investigate the active secondary metabolites of coral derived fungi Aspergillus flavipes. Methods Compounds were isolated and purified by means of various chromatographic techniques,including Silica gel column chromatography,Sephadex LH-20 chromatography and HPLC.The structure of the compounds was identified by NMR combined with the data reported.The nuclear factor kappa B receptor activating factor ligand (RANKL) was used to induce bone marrow macrophage cells (BMMs) differentiate into mature osteoclasts.The tartrate-resistant acid phosphatase (TRAP) specific staining was used to test the inhibitory activity of compounds on osteoclast differentiation. Results Four cytochalasins were isolated from the fungus and their structures were identified as trichalasins H,aspergilluchalasin,aspochalasin I and aspochalasin D.Compounds 3 and 4 showed inhibitory activity on osteoclast differentiation. Conclusion This was the first report of inhibiting osteoclast differentiation activity of compounds 3 and 4.These two compounds might have great significance in the study of new anti-osteoporosis drugs.
  • [1] HOU X M,XU R F,GU Y C,et al.Biological and chemical diversity of coral-derived microorganisms[J].Curr Med Chem,2015,22(32):3707-3762.
    [2] ZHU W M,WANG J F.A review on studies of secondary metabolites from marine fungi[J].Mycosystema,2011,30(2):218-228.
    [3] 宋双,许佳怡.海洋来源的曲霉属真菌次级代谢产物及其活性研究进展[J].中山大学研究生学刊:自然科学与医学版,2015(3):20-32.
    [4] ROCHFORT S,FORD J,OVENDEN S,et al.A novel aspochalasin with HIV-1 integrase inhibitory activity from Aspergillus flavipes[J].J Antibiot,2005,58(4):279-283.
    [5] ZHOU G X,WIJERATNE E M K,BIGELOW D,et al.Aspochalasins I,J,and K:Three new cytotoxic cytochalasans of Aspergillus flavipes from the Rhizosphere of Ericameria laricifolia of the Sonoran desert1[J].J Nat Prod,2004,67(3):328-332.
    [6] BARROW C J,SUN H H.Spiroquinazoline,a novel substance P inhibitor with a new carbon skeleton,isolated from Aspergillus flavipes[J].J Nat Prod,1994,57(4):471-476.
    [7] KWON YJ,MJ,CJ,et al.Flavimycins A and B,dimeric 1,3-dihydroisobenzofurans with peptide deformylase inhibitory activity from Aspergillus flavipes[J].J Nat Prod,2012,75(2):271-274.
    [8] EL AMRANI M,LAI D,DEBBAB A,et al.Protein kinase and HDAC inhibitors from the endophytic fungus Epicoccum nigrum[J].J Nat Prod,2014,77(1):49-56.
    [9] RUKACHAISIRIKUL V,RUNGSAIWATTANA N,KLAIKLAY S,et al.γ-Butyrolactone,cytochalasin,cyclic carbonate,eutypinic acid,and phenalenone derivatives from the soil fungus Aspergillus sp.PSU-RSPG185[J].J Nat Prod,2014,77(11):2375-2382.
    [10] CHEN L,LIU Y T,SONG B,et al.Stereochemical determination of new cytochalasans from the plant endophytic fungus Trichoderma gamsii.[J].Fitoterapia,2014,96(3):115-122.
    [11] CHOO S J,YUN B S,RYOO I J,et al.Aspochalasin I,a melanogenesis inhibitor from Aspergillus sp.[J].J Microbiol Biotechnol,2009,19(4):368-371.
    [12] TOMIKAWA T,SHIN-YA K,KINOSHITA T,et al.Selective cytotoxicity and stereochemistry of aspochalasin D[J].J Antibiot,2001,54(4):379-381.
    [13] ZHANG Y,WANG T,PEI Y,et al.Aspergillin PZ,a novel isoindole-alkaloid from Aspergillus awamori[J].J Antibiot,2002,55(8):693-695.
    [14] IWAMOTO C,YAMADA T,ITO Y,et al.Cytotoxic cytochalasans from a Penicillium,species separated from a marine alga[J].Tetrahedron,2001,57(15):2997-3004.
    [15] QUANG D N,STADLER M,FOURNIER J,et al.Carneic acids A and B,chemotaxonomically significant antimicrobial agents from the xylariaceous ascomycete Hypoxylon carneum[J].J Nat Prod,2006,69(8):1198-1202.
    [16] ALVI K A,NAIR B,PU H,et al.Phomacins:Three novel antitumor cytochalasan constituents produced by a Phoma sp.[J].J Org Chem,1997,62(7):2148-2151.
    [17] ZHANG Y,TIAN R,LIU S,et al.Alachalasins A-G,new cytochalasins from the fungus Stachybotrys charatum[J].Bioorg Med Chem,2009,16(5):2627-2634.
    [18] SI Y,TANG M,LIN S,et al.Cytotoxic cytochalasans from Aspergillus flavipes PJ03-11 by OSMAC method[J].Tetrahedron Letters,2018,59(18):1767-1771.
    [19] ZHAI Z J,LI H W,LIU G W,et al.Andrographolide suppresses RANKL-induced osteoclastogenesis in vitro and prevents inflammatory bone loss in vivo[J].Br J Pharmacol,2014,171(3):663-675.
    [20] KIM J Y,CHEON Y H,KWAK S C,et al.Emodin regulates bone remodeling by inhibiting osteoclastogenesis and stimulating osteoblast formation[J].J Bone Miner Res,2014,29(7):1541-1553.
  • [1] 刘汝雄, 杨万镇, 涂杰, 盛春泉.  铁死亡调控蛋白GPX4的小分子抑制剂研究进展 . 药学实践与服务, 2024, 42(9): 375-378. doi: 10.12206/j.issn.2097-2024.202312075
    [2] 刘丽艳, 余小翠, 孙传铎.  纳武利尤单抗治疗非小细胞肺癌有效性及安全性的Meta分析 . 药学实践与服务, 2024, 42(10): 451-456. doi: 10.12206/j.issn.2097-2024.202310044
    [3] 迟文雅, 袁艳, 李伟林, 吴茼妤, 俞媛.  负载骨髓间充质干细胞/白藜芦醇脂质体的水凝胶支架用于创伤性脑损伤治疗 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202406034
    [4] 宋雨桐, 夏德润, 顾珩, 唐少文, 易洪刚, 沃红梅.  帕博利珠单抗与铂类化疗方案在晚期非小细胞肺癌一线治疗中的药物经济学评价 . 药学实践与服务, 2024, 42(8): 334-340. doi: 10.12206/j.issn.2097-2024.202303023
    [5] 冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军.  雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
    [6] 修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星.  全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 291-296. doi: 10.12206/j.issn.2097-2024.202312054
    [7] 杨媛媛, 安晓强, 许佳捷, 江键, 梁媛媛.  正极性驻极体联合5-氟尿嘧啶对瘢痕成纤维细胞生长抑制的协同作用 . 药学实践与服务, 2024, 42(6): 244-247. doi: 10.12206/j.issn.2097-2024.202310027
    [8] 姜涛, 徐卫凡, 蒋益萍, 夏天爽, 辛海量.  巴戟天丸组方对Aβ损伤成骨细胞的作用及基于网络药理学的机制研究 . 药学实践与服务, 2024, 42(7): 285-290, 296. doi: 10.12206/j.issn.2097-2024.202305011
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  3660
  • HTML全文浏览量:  376
  • PDF下载量:  396
  • 被引次数: 0
出版历程
  • 收稿日期:  2018-11-04
  • 修回日期:  2019-03-04

珊瑚共附生黄柄曲霉次级代谢产物研究

doi: 10.3969/j.issn.1006-0111.2019.02.010
  • 1. 海军军医大学药学院 上海 200433;陆军军医大学士官学校, 河北 石家庄 050000
  • 2. 海军军医大学药学院 上海 200433
  • 3. 安徽中医药大学, 安徽 合肥 230000
    • 基金项目:  国家自然科学基金（41576157）
  • 收稿日期: 2018-11-04
  • 修回日期: 2019-03-04

摘要: 目的 研究东沙短足软珊瑚（Cladiella sp.）来源的黄柄曲霉菌（Aspergillus flavipes）中的活性次级代谢产物。 方法 采用硅胶柱层析、凝胶柱层析、制备HPLC等分离手段对真菌发酵液乙酸乙酯提取物进行分离，运用现代波谱技术结合文献报道数据，对化合物的结构进行鉴定；采用核因子κB受体活化因子配基（RANKL）诱导小鼠骨髓单核巨噬细胞（bone marrow macrophage cells，BMMs）分化为成熟的破骨细胞，经抗酒石酸酸性磷酸酶（TRAP）特异性染色，对化合物抑制破骨细胞分化活性进行研究。 结果 从该株真菌中分离得到4个细胞松弛素类化合物，其结构鉴定为trichalasins H，aspergilluchalasin，aspochalasin I和aspochalasin D。体外活性测试结果显示，化合物3和4可不同程度抑制BMMs向破骨细胞分化。 结论 对化合物3和4抑制破骨细胞分化活性的报告是本文首次报道，对新型抗骨质疏松活性物质研究具有科学价值。

English Abstract

王洪亮, 姜文丽, 李冉, 许聪聪, 张文. 珊瑚共附生黄柄曲霉次级代谢产物研究[J]. 药学实践与服务, 2019, 37(2): 151-155. doi: 10.3969/j.issn.1006-0111.2019.02.010
引用本文: 王洪亮, 姜文丽, 李冉, 许聪聪, 张文. 珊瑚共附生黄柄曲霉次级代谢产物研究[J]. 药学实践与服务, 2019, 37(2): 151-155. doi: 10.3969/j.issn.1006-0111.2019.02.010
shu
WANG Hongliang, JIANG Wenli, LI Ran, XU Congcong, ZHANG Wen. Study on the secondary metabolites of Aspergillus flavipes isolated from coral[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 151-155. doi: 10.3969/j.issn.1006-0111.2019.02.010
Citation: WANG Hongliang, JIANG Wenli, LI Ran, XU Congcong, ZHANG Wen. Study on the secondary metabolites of Aspergillus flavipes isolated from coral[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(2): 151-155. doi: 10.3969/j.issn.1006-0111.2019.02.010
shu

    全文HTML

参考文献 (20)

目录

    /

    返回文章
    返回

    版权所有：《药学实践与服务》编辑委员会

    主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

    电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

    网站备案号 沪ICP备05003363号-1

    本系统由 北京仁和汇智信息技术有限公司 开发    百度统计