<sup>19</sup>F核磁共振定量法测定含氟漱口液中氟化钠的含量

华俊杰; 朱海菲; 李育

doi:10.3969/j.issn.1006-0111.2019.06.009

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

¹⁹F核磁共振定量法测定含氟漱口液中氟化钠的含量

华俊杰, 朱海菲, 李育

文章导航 > 药学实践与服务 > 2019 > 37(6): 518-520

华俊杰, 朱海菲, 李育. 19F核磁共振定量法测定含氟漱口液中氟化钠的含量[J]. 药学实践与服务, 2019, 37(6): 518-520. doi: 10.3969/j.issn.1006-0111.2019.06.009

引用本文:

华俊杰, 朱海菲, 李育. ¹⁹F核磁共振定量法测定含氟漱口液中氟化钠的含量[J]. 药学实践与服务, 2019, 37(6): 518-520. doi: 10.3969/j.issn.1006-0111.2019.06.009

HUA Junjie, ZHU Haifei, LI Yu. Determination of sodium fluoride in fluoride-containing mouthwash by 19F-NMR[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 518-520. doi: 10.3969/j.issn.1006-0111.2019.06.009

Citation:

HUA Junjie, ZHU Haifei, LI Yu. Determination of sodium fluoride in fluoride-containing mouthwash by ¹⁹F-NMR[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(6): 518-520. doi: 10.3969/j.issn.1006-0111.2019.06.009

¹⁹F核磁共振定量法测定含氟漱口液中氟化钠的含量

doi: 10.3969/j.issn.1006-0111.2019.06.009

海军军医大学药学院上海 200433

Determination of sodium fluoride in fluoride-containing mouthwash by ¹⁹F-NMR

School of Pharmacy, Naval Medical University, Shanghai 200433, China

摘要: 目的建立¹⁹F核磁共振技术测定含氟漱口液中氟化钠含量的方法。方法采用¹⁹F核磁共振技术，以三氟乙酸作为内标物，使用300MHz核磁共振波谱仪，在25℃下采集样品的¹⁹F核磁共振谱，建立内标标准曲线，用于氟化钠定量分析。结果氟化钠在0.1~0.8 mg/ml范围内，线性关系良好，回归方程为A=1.1392C-0.0013，r=0.99995；低、中、高3个浓度的平均回收率分别为99.36%、99.31%、99.65%，RSD分别为0.51%、0.35%、0.54%，含氟漱口液中氟化钠的平均含量为99.30%。结论研究建立的¹⁹F核磁共振方法准确度、精密度较好，能够快速、准确地测定含氟漱口液中氟化钠的含量。
- ¹⁹F-NMR /
- 氟化钠 /
- 含量测定 /
- 定量核磁共振
Abstract: Objective To establish a method for the determination of sodium fluoride in fluoride-containing mouthwash by ¹⁹F-NMR technique. Methods The ¹⁹F-NMR technique was used to establish the relevant standard curve with trifluoroacetic acid as the internal standard.The ¹⁹F-NMR spectrum of the sample was collected at 25℃ using a 300 MHz NMR spectrometer. Results The linear relationship of sodium fluoride was good in the range of 0.1-0.8 mg/ml.The regression equation was A=1.1392C-0.0013,r=0.99995.The average recovery of low,medium and high concentration levels were 99.36%,99.31.% and 99.65%,respectively,and RSD was 0.51%,0.35% and 0.54% respectively.The average content of sodium fluoride in the fluoride mouthwash was 99.30%. Conclusion The method was accurate and precise which could be used to determine the sodium fluoride in fluoride-containing mouthwash.
- ¹⁹F-NMR /
- sodium fluoride /
- determination of content /
- standard curve

[1]	国家药典委员会.中华人民共和国药典2015年版四部[S].北京:中国医药科技出版社,2015:441.
[2]	The United States Pharmacopeial Convention.U.S.Pharmacopeia 40-National Formulaty 35[S].The United States Pharmacopeial Convention.2017:761.
[3]	European Directorate for the Quality of Medicines & HealthCare.European Pharmacopoecia 9.0 Index[S].EDQM 2017:54.
[4]	WEBSTER G K,KUMAR S.Expanding the analytical toolbox:pharmaceutical application of quantitative NMR[J].Anal Chem,2014,86(23):11474-11480.
[5]	PAULI G F,CHEN S N,SIMMLER C,et al.Importance of purity evaluation and the potential of quantitative ¹H NMR as a purity assay[J].J Med Chem,2014,57(22):9220-9231.
[6]	HOLZGRABE U,MALET-MARTINO M.Analytical challenges in drug counterfeiting and falsification-The NMR approach[J].J Pharm Biomed Anal,2011,55(4):679-687.
[7]	SANTOS PIMENTA L P,SCHILTHUIZEN M,VERPOORTE R,et al.Quantitative analysis of amygdalin and prunasin in Prunus serotina Ehrh.using ¹H-NMR spectroscopy[J].Phytochem Anal,2014,25(2):122-126.
[8]	WEBER M,HELLRIEGEL C,RÜCK A,et al.Using high-performance quantitative NMR (HP-qNMR®) for certifying traceable and highly accurate purity values of organic reference materials with uncertainties <0.1%[J].Accred Qual Assur,2013,18(2):91-98.
[9]	BEKIROGLU S,MYRBERG O,OSTMAN K,et al.Validation of a quantitative NMR method for suspected counterfeit products exemplified on determination of benzethonium chloride in grapefruit seed extracts[J].J Pharm Biomed Anal,2008,47(4-5):958-961.
[10]	吴迪,陈华,王腾,等.¹⁹F核磁共振定量法测定吉非替尼的含量[J].中国新药杂志,2017,26(19):2343-2346.
[11]	李宏伟,张红芹,郝海军.核磁共振定量法测定恩曲他滨绝对含量[J].化学试剂,2018,40(8):755~758.
[12]	ZHANG FF,JIANG M H,SUN L L,et al.Quantitative analysis of sitagliptin using the ¹⁹F-NMR method:a universal technique for fluorinated compound detection[J].Analyst,2015,140(1):280-286.
[13]	DO N M,OLIVIER M A,SALISBURY JJ,et al.Application of quantitative ¹⁹F and ¹H NMR for reaction monitoring and in situ yield determinations for an early stage pharmaceutical candidate[J].Anal Chem,2011,83(22):8766-8771.
[14]	张卫国,殷忠,郭华,等.用氟离子选择电极测定牙膏中游离氟的影响因素[J].中国地方病学杂志,2007,26(6):693-694.

[1]	桂明珠, 李静, 李志玲. 儿童伏立康唑的血药浓度与CYP2C19、CYP2C9和CYP3A5基因多态性的相关性研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202402020
[2]	王晓飞, 张颖, 顾佳钰, 胡馨儿, 张海, 曹岩. 表面等离子共振传感器的识别元件在医药领域中的研究应用进展 . 药学实践与服务, 2024, 42(): 1-9. doi: 10.12206/j.issn.2097-2024.202309014
[3]	陈炳辰, 佟达丰, 万苗, 闫飞虎, 姚建忠. UPLC-MS/MS法测定小鼠血浆中紫杉醇脂肪酸酯前药及其药代动力学研究 . 药学实践与服务, 2024, 42(8): 341-345. doi: 10.12206/j.issn.2097-2024.202404082
[4]	顾佳钰, 胡馨儿, 王晓飞, 张颖, 张海, 曹岩. 侧流免疫层析定量检测方法的研究进展 . 药学实践与服务, 2024, 42(7): 273-277, 284. doi: 10.12206/j.issn.2097-2024.202307037
[5]	姚小静, 计佩影, 陆峰, 施国荣, 傅翔. 表面增强拉曼光谱法快速测定尿液中曲马多的研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202401072
[6]	陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰. HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(9): 402-406. doi: 10.12206/j.issn.2097-2024.202403030
[7]	李丹, 戴贤春, 王芳珍, 陈奕含, 杨萍, 刘继勇. HPLC-MS/MS测定当归六黄汤中4种不同成分的含量 . 药学实践与服务, 2024, 42(6): 248-252, 266. doi: 10.12206/j.issn.2097-2024.202305007
[8]	夏哲炜, 曾垣烨, 朱海菲, 李育, 陈啸飞. 核磁共振磷谱法测定磷酸氢钙咀嚼片中药物含量 . 药学实践与服务, 2024, 42(9): 399-401, 406. doi: 10.12206/j.issn.2097-2024.202404063

点击查看大图

计量

文章访问数: 3704
HTML全文浏览量: 385
PDF下载量: 827
被引次数: 0

¹⁹F核磁共振定量法测定含氟漱口液中氟化钠的含量

doi: 10.3969/j.issn.1006-0111.2019.06.009

海军军医大学药学院上海 200433

收稿日期: 2019-04-12
修回日期: 2019-07-20

关键词:

¹⁹F-NMR /

氟化钠 /

含量测定 /

定量核磁共振

摘要: 目的建立¹⁹F核磁共振技术测定含氟漱口液中氟化钠含量的方法。方法采用¹⁹F核磁共振技术，以三氟乙酸作为内标物，使用300MHz核磁共振波谱仪，在25℃下采集样品的¹⁹F核磁共振谱，建立内标标准曲线，用于氟化钠定量分析。结果氟化钠在0.1~0.8 mg/ml范围内，线性关系良好，回归方程为A=1.1392C-0.0013，r=0.99995；低、中、高3个浓度的平均回收率分别为99.36%、99.31%、99.65%，RSD分别为0.51%、0.35%、0.54%，含氟漱口液中氟化钠的平均含量为99.30%。结论研究建立的¹⁹F核磁共振方法准确度、精密度较好，能够快速、准确地测定含氟漱口液中氟化钠的含量。