6种黄酮化合物对大鼠肝星状细胞胶原合成的抑制作用
Inhibitory effects of six flavonoids on collagen synthesis of rat hepatic stellate cells
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摘要: 目的 :研究黄颜木素、槲皮素、芹菜素、根皮素、橙皮素和查尔酮对血清、巨噬细胞培养上清液和转化生长因子β1刺激的大鼠肝星状细胞HSC-T6胶原合成的影响。 方法 :3H-脯氨酸掺入法测定细胞胶原合成。 结果 :黄颜木素、槲皮素、芹菜素、根皮素、橙皮素和查尔酮(12.5~50μmol/L)以浓度依赖方式抑制血清、巨噬细胞上清和转化生长因子β1诱导的胶原合成。 结论 :黄颜木素、槲皮素、芹菜素、根皮素、橙皮素和查尔酮具有抑制肝星状细胞胶原合成的作用,可能具有潜在的肝纤维化治疗作用。Abstract: Objective : To study the effects of six flavonoids (fisetin, quercetin, apigenin, phloretin, hesperectin, and chalcone)on collagen synthesis of immortallized rat hepatic stellate cell line HSC-T6 cells. Methods : Collagen synthesis was determined by 3H-proline incorporation. Results :Fisetin, quercetin, apigenin, phloretin, hesperectin, and chalcone (12.5-50μmol/L)concentration-dependently diminished collagen synthesis stimulated by serum, macrophage conditioned medium (MCM)and transforming growth factorβ1(TGFβ1). Conclusion :The six flavonoids inhibited the collagen synthesis of hepatic stellate cells, which may have potential therapeutic effects against liver fibrosis.
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Key words:
- flavonoid /
- hepatic stellate cells /
- collagen /
- macrophages /
- transforming growth factor
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