测定通脉灵片中丹参素含量
Determination of the przewatanshinquinone in Tongmailing tabeles
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摘要: 目的: 建立通脉灵片中丹参素的含量测定方法。 方法: 采用KromasilTM C18柱(200 mm×4.6 mm,5μm ),流动相为甲醇-乙腈-0.045 mol/L磷酸溶液(10:4:86),检测波长281 nm。 结果: 丹参素的线性范围为0.194~2.129μg,r=0.999 8。平均加样回收率为99.56%,RSD为0.84%(n=5)。 结论: 该方法灵敏、简便、重现性好,可用于该制剂的质量控制。Abstract: Objective:To establish a HPLC method of the determination of the przewatanshinquinone in Tongmailing tabeles. Methods: The determination was performed by Re-HPLC on KromasilTM C18 column(200 mm×4.6 mm,5μm)using methanol-toni- trile-0.045 mol/L H3 PO4(10:4:86)as mobile phase,the detection wavelength was 281 nm. Results: The linear rang of przewatan- shinquinone was 0.194~2.129μg,r=0.999 8.The average recovery was 99.56%,RSD was 0.84%(n=5). Conclusion: The method is sensitive,simple,and good reproducibility,which can be applied to the quality control of the preparation.
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Key words:
- Tongmailing tabeles /
- przewatanshinquinone /
- HPLC
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