丹参茎、叶中丹酚酸B的HPLC法比较分析

李正国; 石俊英; 张中湖

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丹参茎、叶中丹酚酸B的HPLC法比较分析

李正国, 石俊英, 张中湖

文章导航 > 药学实践与服务 > 2007 > (5): 307-309

李正国, 石俊英, 张中湖. 丹参茎、叶中丹酚酸B的HPLC法比较分析[J]. 药学实践与服务, 2007, (5): 307-309.

引用本文:

李正国, 石俊英, 张中湖. 丹参茎、叶中丹酚酸B的HPLC法比较分析[J]. 药学实践与服务, 2007, (5): 307-309.

LI Zheng-guo, SHI Jun-ying, ZHANG Zhong-hu. Comparative analysis of salvianolic acid B in Danshen leaves and stems by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 307-309.

Citation:

LI Zheng-guo, SHI Jun-ying, ZHANG Zhong-hu. Comparative analysis of salvianolic acid B in Danshen leaves and stems by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 307-309.

丹参茎、叶中丹酚酸B的HPLC法比较分析

1.
山东中医药大学,山东济南250014;济宁市药品检验所,山东济宁272025
2.
山东中医药大学,山东济南250014
3.
山东省药品检验所,山东济南250018

Comparative analysis of salvianolic acid B in Danshen leaves and stems by HPLC

1.
Shandong University of Traditional Chinese Medicine, Jinan 250014, China;Jining Institute for Drug Control, Jining 271025, China
2.
Shandong University of Traditional Chinese Medicine, Jinan 250014, China
3.
Shandong Institute for Drug Control, Jinan 250018, China

摘要: 目的:建立丹参茎、叶中丹酚酸B的HPLC测定方法,比较不同品种、不同产地、不同采收期丹参茎、叶中丹酚酸B的含量,为进一步合理开发利用丹参茎、叶资源提供理论依据。方法:采用phenomenexC₁₈柱(250mm×4.6 mm,5μm);流动相为甲醇-乙腈-0.25%磷酸溶液(30:10:60);流速为1.0 mL/min;检测波长为286 nm。结果:建立了一个较好的分离和检测条件。工作曲线在(0.488-8.54μg)范围内呈良好的线性关系;平均加样回收率为98.17%。结论:该方法成功用于丹参茎、叶中丹酚酸B的含量测定。不同品种之间丹酚酸B的含量存在差异,其中紫花丹参含量较高。然而对同品种丹参茎叶来说,以泗水产的紫花丹参含量较高,并且8月份采集的含量最高。
- 丹酚酸B /
- 丹参茎叶 /
- HPLC /
- 药物分析
Abstract: Objective : To establish an HPLC method for determination of salvianolic acid B in Danshen leaves and stems,and compare the content of salvianolic acid B in Danshen leaves and stems of different species,different locations and growth stages,so as to supply some evidence for the further exploitation and utilization of Danshen leaves and stems reasonably. Methods : The determination was performed on Phenomenex C₁₈ column(250 mm×4.6 mm 5μm);The mobile phase consisted of acetonitrile-meon-0.25%orthophosphoric acid solution(10∶30∶60) with a flow-rate of 1.0 mL/min.The detecting wavelength was at 286 nm. Results : The calibration curves were good linear regression(r=0.999 9) with measurement ranges(0.488～8.54μg).The recoveries were 98.17%. Conclusions : The method was successfully applied to quantify salvianolic acid B in Danshsen leaves and stems,and salvianolic acid B cotents in Danshsen leaves and stems of different species were not at the same level;Salviae miltiorrhiza Bge contained the higher salvianolic acid B than Salvia miltiorrhiza bge.f.alba.The salvianolic acid B contents in Danshsen leaves and stems from Sishui was the highest in Augest among different locations and growth stages for the same species.
- Salvianolic acid B /
- Danshsen leaves and stems /
- HPLC /
- pharmaceutical analysis

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丹参茎、叶中丹酚酸B的HPLC法比较分析

1. 山东中医药大学,山东济南250014;济宁市药品检验所,山东济宁272025

2. 山东中医药大学,山东济南250014

3. 山东省药品检验所,山东济南250018

收稿日期: 2007-05-11

关键词:

丹酚酸B /

丹参茎叶 /

HPLC /

药物分析

摘要: 目的:建立丹参茎、叶中丹酚酸B的HPLC测定方法,比较不同品种、不同产地、不同采收期丹参茎、叶中丹酚酸B的含量,为进一步合理开发利用丹参茎、叶资源提供理论依据。方法:采用phenomenexC₁₈柱(250mm×4.6 mm,5μm);流动相为甲醇-乙腈-0.25%磷酸溶液(30:10:60);流速为1.0 mL/min;检测波长为286 nm。结果:建立了一个较好的分离和检测条件。工作曲线在(0.488-8.54μg)范围内呈良好的线性关系;平均加样回收率为98.17%。结论:该方法成功用于丹参茎、叶中丹酚酸B的含量测定。不同品种之间丹酚酸B的含量存在差异,其中紫花丹参含量较高。然而对同品种丹参茎叶来说,以泗水产的紫花丹参含量较高,并且8月份采集的含量最高。

English Abstract

Comparative analysis of salvianolic acid B in Danshen leaves and stems by HPLC

1. Shandong University of Traditional Chinese Medicine, Jinan 250014, China;Jining Institute for Drug Control, Jining 271025, China

2. Shandong University of Traditional Chinese Medicine, Jinan 250014, China

3. Shandong Institute for Drug Control, Jinan 250018, China

Received Date: 2007-05-11

Keywords:

Abstract: Objective : To establish an HPLC method for determination of salvianolic acid B in Danshen leaves and stems,and compare the content of salvianolic acid B in Danshen leaves and stems of different species,different locations and growth stages,so as to supply some evidence for the further exploitation and utilization of Danshen leaves and stems reasonably. Methods : The determination was performed on Phenomenex C₁₈ column(250 mm×4.6 mm 5μm);The mobile phase consisted of acetonitrile-meon-0.25%orthophosphoric acid solution(10∶30∶60) with a flow-rate of 1.0 mL/min.The detecting wavelength was at 286 nm. Results : The calibration curves were good linear regression(r=0.999 9) with measurement ranges(0.488～8.54μg).The recoveries were 98.17%. Conclusions : The method was successfully applied to quantify salvianolic acid B in Danshsen leaves and stems,and salvianolic acid B cotents in Danshsen leaves and stems of different species were not at the same level;Salviae miltiorrhiza Bge contained the higher salvianolic acid B than Salvia miltiorrhiza bge.f.alba.The salvianolic acid B contents in Danshsen leaves and stems from Sishui was the highest in Augest among different locations and growth stages for the same species.

李正国, 石俊英, 张中湖. 丹参茎、叶中丹酚酸B的HPLC法比较分析[J]. 药学实践与服务, 2007, (5): 307-309.

引用本文:

李正国, 石俊英, 张中湖. 丹参茎、叶中丹酚酸B的HPLC法比较分析[J]. 药学实践与服务, 2007, (5): 307-309.

LI Zheng-guo, SHI Jun-ying, ZHANG Zhong-hu. Comparative analysis of salvianolic acid B in Danshen leaves and stems by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 307-309.

Citation:

LI Zheng-guo, SHI Jun-ying, ZHANG Zhong-hu. Comparative analysis of salvianolic acid B in Danshen leaves and stems by HPLC[J]. Journal of Pharmaceutical Practice and Service, 2007, (5): 307-309.

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丹参茎、叶中丹酚酸B的HPLC法比较分析

Comparative analysis of salvianolic acid B in Danshen leaves and stems by HPLC

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丹参茎、叶中丹酚酸B的HPLC法比较分析

1. 山东中医药大学,山东济南250014;济宁市药品检验所,山东济宁272025 2. 山东中医药大学,山东济南250014 3. 山东省药品检验所,山东济南250018

English Abstract

Comparative analysis of salvianolic acid B in Danshen leaves and stems by HPLC

1. Shandong University of Traditional Chinese Medicine, Jinan 250014, China;Jining Institute for Drug Control, Jining 271025, China 2. Shandong University of Traditional Chinese Medicine, Jinan 250014, China 3. Shandong Institute for Drug Control, Jinan 250018, China

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目录

1. 山东中医药大学,山东济南250014;济宁市药品检验所,山东济宁272025

2. 山东中医药大学,山东济南250014

3. 山东省药品检验所,山东济南250018

1. Shandong University of Traditional Chinese Medicine, Jinan 250014, China;Jining Institute for Drug Control, Jining 271025, China

2. Shandong University of Traditional Chinese Medicine, Jinan 250014, China

3. Shandong Institute for Drug Control, Jinan 250018, China