反相高效液相色谱法测定乌金胶囊中绿原酸的含量
Determination of chlorogenic acid content in wujin capsule by RP-HPLC
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摘要: 目的 建立反相高效液相色谱法测定乌金胶囊中绿原酸含量,并应用于该制剂质量控制。 方法 使用Agilent extend-C18柱 (4.6 mm×250 mm,5 μm),乙腈-0.1%甲酸(8:92)为流动相,流速1.0 ml/min,柱温30 ℃,检测波长327 nm,测定5批乌金胶囊中绿原酸含量。 结果 绿原酸在2.04 ~102 μg/ml(r=0.999 9)范围内具有良好线性。加样回收率104.68 %,RSD=1.43%(n=6)。5批乌金胶囊中绿原酸含量在55.08~83.31 μg/g之间。 结论 该方法灵敏、准确,适用于乌金胶囊绿原酸含量测定。Abstract: Objective To establish a RP-HPLC method for determination of chlorogenic acid in wujing capsule. Methods Agilent extend-C18 (4.6 mm×250 mm,5 μm)column was used. The mobile phase was acetonitrile: 0.1%formic acid (8:92). The flow rate was 1.0 ml/min, and the temperature was at 35 ℃. The detection wavelength was 327 nm. Determination of chlorogenic acid in wujin capsule of 5 batches of preparations. Results The calibration curve was at the range of 2.04~102 μg/ml(r=0.999 9), and the average recovery was 104.68 %,and RSD was 1.43%(n=6).5 batches of chlorogenic acid contents in wujin capsule were between 55.08~83.31 μg/g. Conclusion The method was sensitive and accurate, and could be used for the quality control of wujin capsule.
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Key words:
- RP-HPLC /
- chlorogenic acid /
- wujin capsule /
- content determination
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