高效液相色谱法测定支扩养阴颗粒中蒙花苷的含量
Determination of buddleoside in Zhikuoyangyin granules by HPLC
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摘要: 目的 建立HPLC测定支扩养阴颗粒中蒙花苷的含量方法.方法 色谱柱为DIKMA Platisil ODS柱 (250 mm×4.6 mm,5μm),流动相为甲醇-水-醋酸(55∶44∶2),流速1.0 ml/min,进样量20μl,检测波长326 nm.结果 蒙花苷在2.95～98.3μg/ml内线性良好,r=0.9999 (n=5),平均回收率100.2%,RSD为0.96%(n=15).结论 该法操作简便、灵敏、可靠,可用于支扩养阴颗粒中蒙花苷的含量测定.Abstract: Objective To establish a high performance liquid chromatography method for the determination of buddleoside in Zhikuoyangyin granules. Methods The HPLC method was performed on a DIKMA Platisil ODS column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of methanol-water-acetic acid (55:44:2). The flow rate was 1.0 ml/min. The sample injection volume was 20 μl. The detective wavelength was 326 nm. Results The calibration curve of buddleoside showed good linear response ranged from 2.95 to 98.3μg/ml with r=0.9999 (n=5). The average recovery of spiked samples for buddleoside was 100.2% while the relative standard deviation for repetitions was 0.96%(n=15). Conclusion The method was sirepie, sensitive,reliable and repeatable,and could be used for the quantitative determination of buddleoside in Zhikuoyangyin granules.
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Key words:
- buddleoside /
- Cirsii herba /
- Zhikuoyangyin granules /
- content determination /
- HPLC
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