中国南海群海绵<em>Agelas mauritiana</em>的化学成分研究

方凤凯; 汤华; 刘宝姝; 庄春林; 孙鹏; 张文

doi:10.3969/j.issn.1006-0111.2015.03.013

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中国南海群海绵Agelas mauritiana的化学成分研究

方凤凯, 汤华, 刘宝姝, 庄春林, 孙鹏, 张文

文章导航 > 药学实践与服务 > 2015 > 33(3): 242-245,274

方凤凯, 汤华, 刘宝姝, 庄春林, 孙鹏, 张文. 中国南海群海绵Agelas mauritiana的化学成分研究[J]. 药学实践与服务, 2015, 33(3): 242-245,274. doi: 10.3969/j.issn.1006-0111.2015.03.013

引用本文:

方凤凯, 汤华, 刘宝姝, 庄春林, 孙鹏, 张文. 中国南海群海绵Agelas mauritiana的化学成分研究[J]. 药学实践与服务, 2015, 33(3): 242-245,274. doi: 10.3969/j.issn.1006-0111.2015.03.013

FANG Fengkai, TANG Hua, LIU Baoshu, ZHUANG Chunlin, SUN Peng, ZHANG Wen. Study on chemical constitunts of sponge Agelas mauritiana from the South China Sea[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 242-245,274. doi: 10.3969/j.issn.1006-0111.2015.03.013

Citation:

FANG Fengkai, TANG Hua, LIU Baoshu, ZHUANG Chunlin, SUN Peng, ZHANG Wen. Study on chemical constitunts of sponge Agelas mauritiana from the South China Sea[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(3): 242-245,274. doi: 10.3969/j.issn.1006-0111.2015.03.013

中国南海群海绵Agelas mauritiana的化学成分研究

doi: 10.3969/j.issn.1006-0111.2015.03.013

第二军医大学药学院海洋药物研究中心, 上海 200433

基金项目: 科技部863项目(2013AA092902),科技部国际合作项目(2014DFG32640),上海市卫生系统优秀学科带头人计划(XBR2013111),上海市优秀学科带头人计划(15XD1504600)

Study on chemical constitunts of sponge Agelas mauritiana from the South China Sea

Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

摘要: 目的对采自南中国海海域的群海绵(Agelas mauritiana)的化学成分进行研究。方法采用硅胶色谱柱、Sephadex LH-20凝胶色谱柱、高效液相色谱等色谱方法,对Agelas mauritiana正丁醇萃取物进行分离纯化;应用现代波谱技术对化合物进行化学结构鉴定;用MTT法对化合物进行体外人肺癌细胞株A549细胞生长抑制活性测试。结果共分离得到8个化合物,分别鉴定为:agelasine A ( 1 )、agelasine B ( 2 )、epi-agelasine C ( 3 )、(-)agelasine D ( 4 )、agelasine E ( 5 )、agelasine F ( 6 )、(-)ageloxime D ( 7 )、aurantiamide acetate ( 8 )。体外活性筛选中,这些化合物对A549显示出不同程度的生长抑制活性,化合物 1 ~ 3 的活性与阳性对照阿霉素相近。结论化合物 1 、 2 、 4 、 5 、 6 、 8 为首次从该种海绵中分离得到。首次选用A549对化合物 1 ~ 7 的活性进行评价,化合物 2 、 3 的显著生长抑制活性为进一步深入研究提供了依据。
- 群海绵 /
- 二萜生物碱 /
- aurantiamideacetate /
- 结构鉴定 /
- 肿瘤生长抑制活性
Abstract: Objective To investigate the chemical constituents of marine sponge Agelas mauritiana collected from the South China Sea. Methods The n-butanol extract of marine sponge Agelas mauritiana was separated and purified by repeated column chromatography on silica gel, Sephadex LH-20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The chemical structures of those obtained compounds were determined on the basis of spectroscopic analysis and comparison with reported data. The tumor cell growth inhibitory activity of these compounds towards human lung carcinoma cells A549 was tested. Results Eight compounds were isolated, including agelasine A ( 1 ), agelasine B ( 2 ), epi-agelasine C ( 3 ), (-)agelasine D ( 4 ), agelasine E ( 5 ), agelasine F ( 6 ), (-)ageloxime D ( 7 ) and aurantiamide acetate ( 8 ). These compounds displayed different level of tumor cell growth inhibitory activity towards cell A549 in vitro. Compounds 1 - 3 showed significant activity towards cell A549, being similar to that of the positive control of adriamycin. Conclusion Compounds 1 , 2 , 4 , 5 , 6 , 8 were isolated for the first time from the sponge Agelas mauritiana. Cell A549 was selected for the first time for the activity evaluation of compounds 1 ~ 7 .Significant inhibition activity of compounds 2 、 3 may hold as a basis for further research.
- Agelas mauritiana /
- diterpene alkaloid /
- aurantiamide acetate /
- structure identification /
- tumor cell growth inhibitory activity

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中国南海群海绵Agelas mauritiana的化学成分研究

doi: 10.3969/j.issn.1006-0111.2015.03.013

第二军医大学药学院海洋药物研究中心, 上海 200433

收稿日期: 2015-03-05
修回日期: 2015-04-16

关键词:

群海绵 /

二萜生物碱 /

aurantiamideacetate /

结构鉴定 /

肿瘤生长抑制活性

摘要: 目的对采自南中国海海域的群海绵(Agelas mauritiana)的化学成分进行研究。方法采用硅胶色谱柱、Sephadex LH-20凝胶色谱柱、高效液相色谱等色谱方法,对Agelas mauritiana正丁醇萃取物进行分离纯化;应用现代波谱技术对化合物进行化学结构鉴定;用MTT法对化合物进行体外人肺癌细胞株A549细胞生长抑制活性测试。结果共分离得到8个化合物,分别鉴定为:agelasine A ( 1 )、agelasine B ( 2 )、epi-agelasine C ( 3 )、(-)agelasine D ( 4 )、agelasine E ( 5 )、agelasine F ( 6 )、(-)ageloxime D ( 7 )、aurantiamide acetate ( 8 )。体外活性筛选中,这些化合物对A549显示出不同程度的生长抑制活性,化合物 1 ~ 3 的活性与阳性对照阿霉素相近。结论化合物 1 、 2 、 4 、 5 、 6 、 8 为首次从该种海绵中分离得到。首次选用A549对化合物 1 ~ 7 的活性进行评价,化合物 2 、 3 的显著生长抑制活性为进一步深入研究提供了依据。

English Abstract

Study on chemical constitunts of sponge Agelas mauritiana from the South China Sea

Research Center for Marine Drugs, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2015-03-05
Rev Recd Date: 2015-04-16

Keywords:

Abstract: Objective To investigate the chemical constituents of marine sponge Agelas mauritiana collected from the South China Sea. Methods The n-butanol extract of marine sponge Agelas mauritiana was separated and purified by repeated column chromatography on silica gel, Sephadex LH-20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The chemical structures of those obtained compounds were determined on the basis of spectroscopic analysis and comparison with reported data. The tumor cell growth inhibitory activity of these compounds towards human lung carcinoma cells A549 was tested. Results Eight compounds were isolated, including agelasine A ( 1 ), agelasine B ( 2 ), epi-agelasine C ( 3 ), (-)agelasine D ( 4 ), agelasine E ( 5 ), agelasine F ( 6 ), (-)ageloxime D ( 7 ) and aurantiamide acetate ( 8 ). These compounds displayed different level of tumor cell growth inhibitory activity towards cell A549 in vitro. Compounds 1 - 3 showed significant activity towards cell A549, being similar to that of the positive control of adriamycin. Conclusion Compounds 1 , 2 , 4 , 5 , 6 , 8 were isolated for the first time from the sponge Agelas mauritiana. Cell A549 was selected for the first time for the activity evaluation of compounds 1 ~ 7 .Significant inhibition activity of compounds 2 、 3 may hold as a basis for further research.