G-CSF减轻大鼠下肢缺血再灌注致肺功能损伤的实验研究
Attenuates pulmonary dysfunction induced by ischaemia-reperfusion of lower extremitates in rats
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摘要: 目的 研究重组粒细胞集落刺激因子(G-CSF)对大鼠下肢缺血再灌注所致肺功能损伤的治疗作用。方法 雄性SD大鼠随机分3组,手术组及给药组麻醉后开腹解剖腹主动脉,在肾动脉水平远端阻断120min,开放、再灌注120min;假手术组不阻断腹主动脉,余同手术组。3组均于术前经舌静脉注入伊文氏蓝 (Evan'sbluedye) 30mg/kg;给药组术前经尾静脉注入G-CSF 20μg/kg,余二组给予生理盐水1ml。术后取肺组织测定丙二醛(malonyldialdehyde,MDA)及伊文氏蓝含量。结果 给药组MDA含量为(1.71±0.34)nmol/mg,假手术组为(1.73±0.65)nmol/mg,均显著低于手术组(2.54±0.39)nmol/mg。给药组伊文氏蓝含量为(1.50±0.2 9)μg/mg,假手术组为(0.13±0.07)μg/mg,均显著低于手术组(3.07±1.18)μg/mg。 结论G-CSF有减轻大鼠下肢缺血再灌注所致肺功能损伤的作用。
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关键词:
- 重组粒细胞集落刺激因子(G-CSF) /
- 缺血再灌注 /
- 肺功能损伤
Abstract: OBJECTIVE To investigate the therapeutic efficacy of Granulocyte colony-stimulating factor (G-CSF) against rat pulmonary dysfunction induced by ischaemia-reperfusion of lower extremities.METHODS Male Spague-Dawley rats were randomised into three group.The operated and G-CSF group underwent laparotomy and clamping of the infrarenal abdominal aorta for 120min followed by 120min of reperfusion; The sham-operated group underwent laparot-my for 240min only;The G-CSF group was pretreated with G-CSF 20μg/kg through iv and the other given 1ml saline each.A Ⅱ groups reeived Evan's blue dye 30mg/kg iv, preoperatively. Concentrations of lung malonyldiaclehyde (MDA) and Evan's blue blye were measured as marker of lung injury. RESZJLTS Lung MDA level of G-CSF group was(1.71±0.34)nmol/mg;Sham-operated group was(1.73±0.65)nmol/mg,both significantly lower than that of operated group(2.54±0.39)nmol/mg. Even's blue dye concentration was significantly higher in operated group(3.07±1.18)μg/mg than in G-CSF group(1.50±0.29)μg/mg and sham-operated group(0.13±0.07)μg/mg. CONCLUSIGN G-CSF has therapeutic efficacy against pulmonary dysfunction which was induced by ischaemia-reperfusion of lower extremitates in rats.-
Key words:
- G-CSF /
- ischaemia-reperfusion /
- pulmonary dysfunction
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