天花粉多糖的微波提取及含量测定
Microwave technique extraction and content determinations of polysaccharide of Snakegourd Root
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摘要: 目的 从天花粉中提取多糖,并测定其含量。方法 运用微波技术用水提醇沉法提取天花粉多糖,用酚-硫酸比色法测定多糖含量。结果 测得天花粉中多糖含量18.3012%,平均回收率为98.86%,RSD=1.68%(n =5)。结论 首次运用微波技术从天花粉中提取出多糖,反应速度大大加快,收率提高。Abstract: OBJECTIVE To extract and determine content polysaccharides of Snakegourd root.METHODS Using microwave technique,polysaccharides of Snakegourd root were obtained by hotwater extraction and ethanol precipitation,and content was determinated with phenol-vitriolic colorimetry.RESULTS The polysaccharides content of Snakegourd root was 18.3012%,Recovery rate was 98.86%.CONCLUSION The polysaccharides of Snakegourd root has been obtained for the first time by microwave technique.
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Key words:
- microwave technique /
- Snakegourd root /
- polysaccharide /
- extraction /
- determination
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