肠康颗粒质量标准的研究
Study on quality control standard of changkang keli
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摘要: 目的 研究建立肠康颗粒的质量标准。 方法 采用薄层色谱法对肠康颗粒中黄连、乌梅进行定性鉴别;采用高效液相色谱法,测定方中君药黄连中盐酸小檗碱的含量。 结果 在薄层色谱中能检出黄连和乌梅;盐酸小檗碱在5.59~178.88 μg/ml范围内,浓度与峰面积线性关系良好(r=0.999 9)。平均加样回收率为:103.3%(RSD=1.26%,n=6)。 结论 建立的方法对方中二味药材能准确、快速地进行定性、定量检测,可用于肠康颗粒的质量控制。Abstract: Objective To establish the quality standard for changkang keli. Methods Coptidis rhizome , mume fructus were identified by TLC. The content of berberine hydrochloride was determined by HPLC. Results Coptidis rhizome, mume fructus could be identified by TLC. Berberine hydrochloride showed a good linear relationship at a rang of 5.59~178.88 μg/ml (r=0.999 9). The average recovery (n=6) was 103.3% (RSD=1.26%). Conclusion The methods were accurate and quick, which could be used for the quality control of changkang keli.
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Key words:
- changkang keli /
- HPLC /
- TLC /
- coptidis rhizome /
- mume fructus /
- berberine hydrochloride
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[1] 中国药典2010版.一部[S].2005:54,213. [2] 王宝琴.中成药质量标准与标准物质研究[M].北京:中国医药科技出版社,1994: 118. [3] 安登魁. 现代药物分析选论[M].北京:中国医药科技出版社,2000:781.
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