摘要:
目的 探讨乌梅对溃疡性结肠炎(UC)小鼠结肠组织过氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的影响。 方法 采用3%葡聚糖硫酸钠(DSS)溶液给小鼠自由饮水,建立小鼠溃疡性结肠炎模型,造模成功后,正常组、模型组给予0.33 ml生理盐水灌胃,乌梅低、中、高剂量组分别按设计剂量灌胃,每天1次,均连续给药10 d,期间进行隐血试验和观察粪便性状以及进行疾病活动度(DAI)评分;肉眼观察结合显微镜观察结肠组织形态学评分;测定病变结肠组织中SOD活性与MDA含量。 结果 发现乌梅中、高剂量组的小鼠无肉眼血便,可见松散大便。乌梅低、中、高剂量组的DAI均降低,中、高剂量组与模型组比较具有极显著性差异(P<0.01),在结肠黏膜病理改变中,乌梅高剂量组黏膜糜烂不严重,溃疡小,黏膜充血、水肿程度轻;在测定的SOD活力值中,中、高剂量组与模型组比较具有极显著性差异(P<0.01),均明显高于模型组。中剂量组与正常组比较具有显著性差异(P<0.05),高剂量组与正常组比较无显著性差异。测得MDA含量中,中、高剂量组与模型组比较具有极显著性差异(P<0.01),均明显低于模型组。 结论 提示自由基与UC的结肠组织损伤密切相关,参考UC的病理过程,乌梅对UC有明显治疗效果,并可能通过提高病变组织的SOD活性与降低MDA含量来发挥治疗作用。
Abstract:
Objective To study the effect of Fructus Mume(FM) on superoxide dismutase(SOD) activity and malondialdehyde(MDA) content in ulcerative colitis in mouse. Methods The mouse model of ulcerative colitis was set up by giving 3% dextran sulfate sodium(DSS) solution to the mouse as free drinking water, the normal group, model group were given 0.33 ml 0.9%Nacl, while FM high, middle and low dose groups were given the designed dosage by intragastric administration once a day, and last for 10 days. The occult blood test, observation of stool and the disease activity score (DAI) were observed. The colonic tissue morphology score was observed by visual inspection combined with microscope;SOD activity and MDA content of the lesions colon tissue were determined. Results The groups of FM high and middle dose had loose stools but no visible blood. DAI of all groups had decreased. There was significant difference (P< 0.01) in DAI between middle and high dose groups with model group. In the colonic mucosal pathological changes, FM high dose group had no severe mucosal erosion, ulcer, mucosal hyperemia and edema degree of light. In the determination of the activity of SOD value, FM high dose group, compared with the model group was significant difference (P< 0.01), higher than that in the model group. FM middle dose group compared with the normal group was significant difference (P< 0.05). There were no significant difference between FM high dose group and normal group. The measured MDA content, FM high dose group compared with the model group was significant difference (P< 0.01), which was lower than that in the model group. Conclusions Free radicals were closely related to UC colonic tissue damage, which participated in UC pathological process. FM had obvious therapeutic effect on UC, and it might play a therapeutic role by enhancing the lesion tissue SOD activity and lower MDA content in UC.
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