麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选

韩琳; 吕超; 李敏; 黄慧梅; 畅婉琳; 彭成成; 柳润辉

doi:10.3969/j.issn.1006-0111.2014.03.012

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麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选

韩琳, 吕超, 李敏, 黄慧梅, 畅婉琳, 彭成成, 柳润辉

文章导航 > 药学实践与服务 > 2014 > 32(3): 209-211,219

韩琳, 吕超, 李敏, 黄慧梅, 畅婉琳, 彭成成, 柳润辉. 麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选[J]. 药学实践与服务, 2014, 32(3): 209-211,219. doi: 10.3969/j.issn.1006-0111.2014.03.012

引用本文:

HAN Lin, LV Chao, LI Min, HUANG Huimei, CHANG Wanlin, PENG Chengcheng, LIU Runhui. Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 209-211,219. doi: 10.3969/j.issn.1006-0111.2014.03.012

Citation:

HAN Lin, LV Chao, LI Min, HUANG Huimei, CHANG Wanlin, PENG Chengcheng, LIU Runhui. Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 209-211,219. doi: 10.3969/j.issn.1006-0111.2014.03.012

麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选

doi: 10.3969/j.issn.1006-0111.2014.03.012

1.
第二军医大学药学院, 上海 200433
2.
福建中医药大学药学院, 福建福州 350108
3.
上海交通大学药学院, 上海 200240

Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill

1.
School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian 350108, China
3.
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

摘要: 目的建立原代心肌细胞缺氧-复氧模型,并对麝香保心丸及其20种血中活性成分进行抗缺氧-复氧损伤的活性筛选。方法取新生(1~3 d)SD乳鼠的心脏,建立原代心肌细胞的缺氧-复氧损伤模型,并采用四甲基偶氮唑盐(MTT)比色法进行麝香保心丸及其入血成分的活性筛选。结果麝香保心丸在50 μg/ml浓度下具有较好的保护原代心肌细胞缺氧-复氧损伤的作用;人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮具有较好的保护原代心肌细胞缺氧-复氧损伤的作用。结论麝香保心丸具有抗心肌细胞缺氧-复氧损伤的作用,人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮是其主要的有效成分。该研究为麝香保心丸深入的药效和机制研究奠定了基础。
- 麝香保心丸 /
- 心肌细胞 /
- 缺氧-复氧模型 /
- 活性成分筛选
Abstract: Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill (SBP) absorbed in blood against hypoxia/reoxygenation injury. Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats(1~3 days old) and were used to build hypoxia/reoxygenation injury model. The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method. Results SBP showed significant protective effect against cardiomyocytes hypoxia/reoxygenation injury atthe concentration of 50 μg/ml. Ginsenoside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect against cardiomyocytes hypoxia/reoxygenation injury. Conclusion SBP have the protective activity against cardiomyocytes hypoxia/reoxygenation injury, and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP. This experiment offered basis for further pharmacodynamics and mechanism study of SBP.
- Shexiang Baoxin Pill (SBP) /
- cardiomyocyte /
- hypoxia/reoxygenation model /
- screening of active components

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麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选

doi: 10.3969/j.issn.1006-0111.2014.03.012

1. 第二军医大学药学院, 上海 200433

2. 福建中医药大学药学院, 福建福州 350108

3. 上海交通大学药学院, 上海 200240

收稿日期: 2014-01-15
修回日期: 2014-04-02

关键词:

摘要: 目的建立原代心肌细胞缺氧-复氧模型,并对麝香保心丸及其20种血中活性成分进行抗缺氧-复氧损伤的活性筛选。方法取新生(1~3 d)SD乳鼠的心脏,建立原代心肌细胞的缺氧-复氧损伤模型,并采用四甲基偶氮唑盐(MTT)比色法进行麝香保心丸及其入血成分的活性筛选。结果麝香保心丸在50 μg/ml浓度下具有较好的保护原代心肌细胞缺氧-复氧损伤的作用;人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮具有较好的保护原代心肌细胞缺氧-复氧损伤的作用。结论麝香保心丸具有抗心肌细胞缺氧-复氧损伤的作用,人参皂苷Rb1、人参皂苷Rb2、蟾毒灵和麝香酮是其主要的有效成分。该研究为麝香保心丸深入的药效和机制研究奠定了基础。

English Abstract

Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill

1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian 350108, China

3. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

Received Date: 2014-01-15
Rev Recd Date: 2014-04-02

Keywords:

Abstract: Objective To build hypoxia/reoxygenation injury model in cultured neonatal rat cardiomyocyte and screen active components from Shexiang Baoxin Pill (SBP) absorbed in blood against hypoxia/reoxygenation injury. Methods Cardiomyocytes were isolated and purified from hearts of neonatal Sprague Dawley rats(1~3 days old) and were used to build hypoxia/reoxygenation injury model. The components of SBP absorbed in blood were screened by methyl thiazolil tetracolium (MTT) colorimetic method. Results SBP showed significant protective effect against cardiomyocytes hypoxia/reoxygenation injury atthe concentration of 50 μg/ml. Ginsenoside Rb1, Rb2, bufalin and muscone of twenty components from SBP absorbed in blood also possessed significant protective effect against cardiomyocytes hypoxia/reoxygenation injury. Conclusion SBP have the protective activity against cardiomyocytes hypoxia/reoxygenation injury, and ginsenoside Rb1, Rb2, bufalin, muscone are the main active components of SBP. This experiment offered basis for further pharmacodynamics and mechanism study of SBP.

引用本文:

Citation:

HAN Lin, LV Chao, LI Min, HUANG Huimei, CHANG Wanlin, PENG Chengcheng, LIU Runhui. Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(3): 209-211,219. doi: 10.3969/j.issn.1006-0111.2014.03.012

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麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选

doi: 10.3969/j.issn.1006-0111.2014.03.012

Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill

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麝香保心丸抗心肌细胞缺氧-复氧损伤活性成分筛选

doi: 10.3969/j.issn.1006-0111.2014.03.012

1. 第二军医大学药学院, 上海 200433 2. 福建中医药大学药学院, 福建 福州 350108 3. 上海交通大学药学院, 上海 200240

English Abstract

Screening of main active components against cardiomyocyte hypoxia/reoxygenation injury in shexiang baoxin Pill

1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China 2. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian 350108, China 3. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China

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目录

1. 第二军医大学药学院, 上海 200433

2. 福建中医药大学药学院, 福建福州 350108

3. 上海交通大学药学院, 上海 200240

1. School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian 350108, China

3. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China