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脑中风是由于脑部血管突然破裂或因血管阻塞导致血液不能流入大脑而引起脑组织损伤的一类疾病[1]。大多数患者在治疗后会遗留不同程度的神经功能缺损,比如偏瘫、嘴歪眼斜、智力障碍、语言认知功能丧失等[2]。因而开发神经恢复剂,修复损伤神经功能尤为重要。修复受损神经功能的途径之一是促进被破坏或受损害的神经重塑[3]。神经重塑通过诱导神经元分化,促进突起向外生长,与其他神经元建立连接,重构皮层,从而修复损伤的神经功能[4]。
黄芪异黄酮类化合物主要包括毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷,结构如图1所示[5]。毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷对急性脑缺血具有神经保护作用,对缺血后脑损伤神经功能的恢复作用未见报道[6-8]。
PC 12细胞是来源于成年大鼠肾上腺髓质嗜铬细胞瘤的细胞系,培养PC 12细胞可动态观察其神经分化的过程[9],因此可将PC 12细胞作为筛选促神经分化、恢复损伤神经功能药物的理想模型。本项研究工作使用PC 12细胞,观察毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷是否具有诱导细胞神经分化的能力。神经生长因子(nerve growth factor, NGF)具有明确的促进神经元分化作用[10],在本研究中用作阳性对照。
The effect of four compounds of astragalus isoflavones on the differentiation of PC 12 cells
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摘要:
目的 探究毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷对PC 12细胞分化的影响。 方法 培养PC 12细胞,与不同浓度的神经生长因子(NGF)、毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷共处理5 d,1次/d,连续3次,观察PC 12细胞突起向外生长的情况。同时,用免疫荧光染色检测上述化合物对β微管蛋白(β III-tubulin)表达的影响。 结果 与对照组相比,毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷和芒柄花苷(0.01~10.00 μmol/L)未能促进PC 12细胞突起向外生长和β微管蛋白的表达。 结论 毛蕊异黄酮、刺芒柄花素、毛蕊异黄酮苷、芒柄花苷不能促进PC 12细胞分化。 Abstract:Objective To investigate the effects of calycosin, formononetin, calycosin-7-glucoside and ononin on PC 12 cells differentiation. Methods PC 12 cells were cultured and treated with different concentrations of nerve growth factor (NGF), calycosin, formononetin, calycosin-7-glucoside and ononin for 5 days, once a day, 3 times in a row. The neurite outgrowth of PC 12 cells was observed and the expression of β III-tubulin were measured by immunofluorescence. Results Compared with the vehicle group, neurite outgrowth and the expression of β III tubulin in PC 12 cells had not promoted by calycosin, formononetin, calycosin-7-glucoside and ononin (0.01-10.00 μmol/L). Conclusion PC 12 cells differentiation could not be induced by calycosin, formononetin, calycosin-7-glucoside and ononin. -
Key words:
- calycosin /
- formononetin /
- calycosin-7-glucoside /
- ononin /
- PC 12 cells /
- cell differentiation
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