正交试验优选元宝草中总黄酮的提取工艺

龚颖珍; 郑承剑; 秦路平

doi:10.3969/j.issn.1006-0111.2015.02.011

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正交试验优选元宝草中总黄酮的提取工艺

龚颖珍, 郑承剑, 秦路平

文章导航 > 药学实践与服务 > 2015 > 33(2): 134-137

龚颖珍, 郑承剑, 秦路平. 正交试验优选元宝草中总黄酮的提取工艺[J]. 药学实践与服务, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

引用本文:

龚颖珍, 郑承剑, 秦路平. 正交试验优选元宝草中总黄酮的提取工艺[J]. 药学实践与服务, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

GONG Yingzhen, ZHENG Chengjian, QIN Luping. Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

Citation:

GONG Yingzhen, ZHENG Chengjian, QIN Luping. Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

正交试验优选元宝草中总黄酮的提取工艺

doi: 10.3969/j.issn.1006-0111.2015.02.011

1.
福建中医药大学药学院, 福建福州 350108;第二军医大学药学院生药学教研室, 上海 200433
2.
第二军医大学药学院生药学教研室, 上海 200433

基金项目: 上海市科委中药现代化专项 (2011DZ1970502)

Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments

1.
School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

摘要: 目的优选元宝草中总黄酮的提取工艺。方法在单因素试验的基础上,考察乙醇浓度、料液比、提取温度等因素对元宝草中总黄酮提取率的影响,并采用L₉(3)⁴正交试验优选最佳提取条件。结果影响元宝草中总黄酮提取率的因素顺序为乙醇浓度>料液比>提取次数>提取温度。元宝草最佳提取工艺条件为70%乙醇,提取温度60 ℃,乙醇用量20倍,提取次数3次。该条件下元宝草中总黄酮平均得率为2.38%。结论本实验设计合理、测定方法可行、实验结果可靠,为大批量从元宝草中提取总黄酮提供了理论依据。
- 元宝草 /
- 总黄酮 /
- 提取工艺 /
- 正交试验
Abstract: Objective To optimize the extraction technology of total falvonoids from Hypericum sampsonii. Methods On the basis of single factor test, solid-liquid ratio, ethanol concentration, extraction time, extraction temperature and extraction times on the extraction rate were studied by taking the content of total falvonoids as indexes. Orthogonal test was conducted to establish the extraction technology of total falvonoids from Hypericum sampsonii. Results The influence degree of factors on the extraction rate of total falvonoids was in the order of ethanol concentration, solid-liquid ratio, extraction times, and temperature.The optimum extraction condition was confirmed as follows: ethanol concentration of 70%, extraction temperature of 60 ℃, and extraction times of 3×1.5 h, solid to liquid ratio of 120. Under this condition,the average extraction rate of total falvonoids reached 2.38%. Conclusion The test was reasonable in design and credible in findings, and was easily operated, which could provide scientific basis for the batch extraction of total flavonoids from Hypericum sampsonii.
- Hypericum sampsonii /
- total flavonoids /
- extracting technology /
- orthogonal test

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[2]	郭澄,郑清明,郑汉臣. 元宝草黄酮类成分的抗抑郁作用研究[J].药学实践杂志,2005,23(6):345-347,361.
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[8]	Chen MT,Chen CM. Xanthones from Hypericum sampsonii[J].Heterocycles,1985, 23(10): 2543-2548.
[9]	郭澄,郑清明,郑汉臣. 元宝草不同药用部位中芒果苷和总黄酮的含量测定[J].分析化学,2006,34(11): 1666.
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正交试验优选元宝草中总黄酮的提取工艺

doi: 10.3969/j.issn.1006-0111.2015.02.011

1. 福建中医药大学药学院, 福建福州 350108;第二军医大学药学院生药学教研室, 上海 200433

2. 第二军医大学药学院生药学教研室, 上海 200433

基金项目: 上海市科委中药现代化专项 (2011DZ1970502)

收稿日期: 2013-10-17
修回日期: 2014-03-17

关键词:

元宝草 /

总黄酮 /

提取工艺 /

正交试验

摘要: 目的优选元宝草中总黄酮的提取工艺。方法在单因素试验的基础上,考察乙醇浓度、料液比、提取温度等因素对元宝草中总黄酮提取率的影响,并采用L₉(3)⁴正交试验优选最佳提取条件。结果影响元宝草中总黄酮提取率的因素顺序为乙醇浓度>料液比>提取次数>提取温度。元宝草最佳提取工艺条件为70%乙醇,提取温度60 ℃,乙醇用量20倍,提取次数3次。该条件下元宝草中总黄酮平均得率为2.38%。结论本实验设计合理、测定方法可行、实验结果可靠,为大批量从元宝草中提取总黄酮提供了理论依据。

English Abstract

Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments

1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2013-10-17
Rev Recd Date: 2014-03-17

Keywords:

Abstract: Objective To optimize the extraction technology of total falvonoids from Hypericum sampsonii. Methods On the basis of single factor test, solid-liquid ratio, ethanol concentration, extraction time, extraction temperature and extraction times on the extraction rate were studied by taking the content of total falvonoids as indexes. Orthogonal test was conducted to establish the extraction technology of total falvonoids from Hypericum sampsonii. Results The influence degree of factors on the extraction rate of total falvonoids was in the order of ethanol concentration, solid-liquid ratio, extraction times, and temperature.The optimum extraction condition was confirmed as follows: ethanol concentration of 70%, extraction temperature of 60 ℃, and extraction times of 3×1.5 h, solid to liquid ratio of 120. Under this condition,the average extraction rate of total falvonoids reached 2.38%. Conclusion The test was reasonable in design and credible in findings, and was easily operated, which could provide scientific basis for the batch extraction of total flavonoids from Hypericum sampsonii.

龚颖珍, 郑承剑, 秦路平. 正交试验优选元宝草中总黄酮的提取工艺[J]. 药学实践与服务, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

引用本文:

龚颖珍, 郑承剑, 秦路平. 正交试验优选元宝草中总黄酮的提取工艺[J]. 药学实践与服务, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

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正交试验优选元宝草中总黄酮的提取工艺

doi: 10.3969/j.issn.1006-0111.2015.02.011

Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments

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正交试验优选元宝草中总黄酮的提取工艺

doi: 10.3969/j.issn.1006-0111.2015.02.011

1. 福建中医药大学药学院, 福建 福州 350108;第二军医大学药学院生药学教研室, 上海 200433 2. 第二军医大学药学院生药学教研室, 上海 200433

English Abstract

Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments

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1. 福建中医药大学药学院, 福建福州 350108;第二军医大学药学院生药学教研室, 上海 200433

2. 第二军医大学药学院生药学教研室, 上海 200433