正交试验优选元宝草中总黄酮的提取工艺

龚颖珍; 郑承剑; 秦路平

doi:10.3969/j.issn.1006-0111.2015.02.011

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

《药学实践与服务》杂志目前不收取审稿费、版面费、加急费等费用，如收到邮件声称是编辑部X编辑，要求加作者微信的，请谨防财产损失！编辑部用于作者校对时绑定微信的邮件通过yxsjzzs@163.com发送，标题是《药学实践与服务》XML数字出版服务微信绑定，请区分开。

正交试验优选元宝草中总黄酮的提取工艺

龚颖珍, 郑承剑, 秦路平

文章导航 > 药学实践与服务 > 2015 > 33(2): 134-137

龚颖珍, 郑承剑, 秦路平. 正交试验优选元宝草中总黄酮的提取工艺[J]. 药学实践与服务, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

引用本文:

龚颖珍, 郑承剑, 秦路平. 正交试验优选元宝草中总黄酮的提取工艺[J]. 药学实践与服务, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

GONG Yingzhen, ZHENG Chengjian, QIN Luping. Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

Citation:

GONG Yingzhen, ZHENG Chengjian, QIN Luping. Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

正交试验优选元宝草中总黄酮的提取工艺

doi: 10.3969/j.issn.1006-0111.2015.02.011

1.
福建中医药大学药学院, 福建福州 350108;第二军医大学药学院生药学教研室, 上海 200433
2.
第二军医大学药学院生药学教研室, 上海 200433

基金项目: 上海市科委中药现代化专项 (2011DZ1970502)

Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments

1.
School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
2.
Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

摘要: 目的优选元宝草中总黄酮的提取工艺。方法在单因素试验的基础上,考察乙醇浓度、料液比、提取温度等因素对元宝草中总黄酮提取率的影响,并采用L₉(3)⁴正交试验优选最佳提取条件。结果影响元宝草中总黄酮提取率的因素顺序为乙醇浓度>料液比>提取次数>提取温度。元宝草最佳提取工艺条件为70%乙醇,提取温度60 ℃,乙醇用量20倍,提取次数3次。该条件下元宝草中总黄酮平均得率为2.38%。结论本实验设计合理、测定方法可行、实验结果可靠,为大批量从元宝草中提取总黄酮提供了理论依据。
- 元宝草 /
- 总黄酮 /
- 提取工艺 /
- 正交试验
Abstract: Objective To optimize the extraction technology of total falvonoids from Hypericum sampsonii. Methods On the basis of single factor test, solid-liquid ratio, ethanol concentration, extraction time, extraction temperature and extraction times on the extraction rate were studied by taking the content of total falvonoids as indexes. Orthogonal test was conducted to establish the extraction technology of total falvonoids from Hypericum sampsonii. Results The influence degree of factors on the extraction rate of total falvonoids was in the order of ethanol concentration, solid-liquid ratio, extraction times, and temperature.The optimum extraction condition was confirmed as follows: ethanol concentration of 70%, extraction temperature of 60 ℃, and extraction times of 3×1.5 h, solid to liquid ratio of 120. Under this condition,the average extraction rate of total falvonoids reached 2.38%. Conclusion The test was reasonable in design and credible in findings, and was easily operated, which could provide scientific basis for the batch extraction of total flavonoids from Hypericum sampsonii.
- Hypericum sampsonii /
- total flavonoids /
- extracting technology /
- orthogonal test

[1]	国家中医药管理局《中华本草》编委会.中华本草[M]. 上海:上海科学技术出版社,1999:605.
[2]	郭澄,郑清明,郑汉臣. 元宝草黄酮类成分的抗抑郁作用研究[J].药学实践杂志,2005,23(6):345-347,361.
[3]	康佳敏,欧阳胜,肖炳坤,等. 元宝草化学成分及药理作用的研究[J].科学技术与工程,2011,11(14):3248-3252,3262.
[4]	Xin WB, Mao ZJ, Jin GL, et al. Two new xanthones from Hypericum sampsonii and biological activity of the isolated compounds[J].Phytother Res,2011, 25(4): 536-539.
[5]	Xin WB, Jin GL, Mao ZJ, et al. Two unusual phenolic substances and one new xanthone from Hypericum sampsonii[J].Helvetica Chim Acta, 2011,94(4):686-692.
[6]	傅芃,张川,张卫东,等. 呫吨酮类化合物的药理活性研究进展[J].药学实践杂志,2005,23(1): 6-12.
[7]	郑清明.元宝草及同属植物的生药学研究[D].第二军医大学,2005.
[8]	Chen MT,Chen CM. Xanthones from Hypericum sampsonii[J].Heterocycles,1985, 23(10): 2543-2548.
[9]	郭澄,郑清明,郑汉臣. 元宝草不同药用部位中芒果苷和总黄酮的含量测定[J].分析化学,2006,34(11): 1666.
[10]	石金城,闫显光,刘媛,等. 元宝草抗抑郁活性部位筛选研究[J].辽宁中医药大学学报,2010,12(5): 7-9.
[11]	郑子敏,韦健全,罗莹,等. 芒果苷对行为绝望小鼠的抗抑郁作用[J].时珍国医国药,2009,20(4):978-979.

[1]	彭莹, 刘欣, 聂依文, 王歆荷, 年华, 朱建勇. 三种狼毒乙醇提取物对咪喹莫特诱导的银屑病小鼠防治作用研究 . 药学实践与服务, 2025, 43(): 1-6. doi: 10.12206/j.issn.2097-2024.202406029
[2]	向兴, 张慧卿, 张琪金, 刘银沁, 黄宝康. 飞机草中医药文献研究及其中药性能探讨 . 药学实践与服务, 2025, 43(4): 1-5. doi: 10.12206/j.issn.2097-2024.202410019
[3]	江冼芮, 段雅倩, 刘畅, 张成中. 淫羊藿中黄酮苷类化合物的群体感应抑制作用研究 . 药学实践与服务, 2025, 43(4): 1-5. doi: 10.12206/j.issn.2097-2024.202409060
[4]	杨贺英, 罗彩萍, 彭婷, 梁文仪, 沈颂章, 苏娟. 花椒生物碱富集纯化工艺优化及其成分分析 . 药学实践与服务, 2025, 43(2): 75-81. doi: 10.12206/j.issn.2097-2024.202404066
[5]	周丽城, 欧已铭, 王园. 玉米须黄酮化学成分与药理作用研究进展 . 药学实践与服务, 2025, 43(2): 51-58. doi: 10.12206/j.issn.2097-2024.202309037
[6]	续畅, 周心娜, 漆璐, 王瑜, 王兴河. 基于文献计量学对我国临床试验用药品管理研究现状的数据挖掘 . 药学实践与服务, 2025, 43(): 1-6. doi: 10.12206/j.issn.2097-2024.202404050
[7]	乔方良, 蒋益萍, 夏天爽, 刘爱军, 赵凯, 辛海量. 对萼猕猴桃苷E提取分离纯化工艺的研究 . 药学实践与服务, 2025, 43(): 1-7. doi: 10.12206/j.issn.2097-2024.202407001
[8]	陈方剑, 赵娟娟, 叶侃倜, 孙煜昕, 刘继勇, 杨骏. 血通胶囊提取工艺优化及质量控制研究 . 药学实践与服务, 2025, 43(2): 82-86, 91. doi: 10.12206/j.issn.2097-2024.202409003
[9]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055
[10]	张成中, 朱雪艳, 卜其涛, 王宏瑞, 黄宝康. 基于网络药理学与分子对接预测鸡骨草特征图谱研究 . 药学实践与服务, 2024, 42(8): 350-358. doi: 10.12206/j.issn.2097-2024.202303048
[11]	凯丽比努尔·奥布力艾散, 李倩, 谢志, 贾文彦, 尹东锋. 星点设计-效应面法优化仑伐替尼混合胶束的制备工艺 . 药学实践与服务, 2024, 42(11): 495-502. doi: 10.12206/j.issn.2097-2024.202403019
[12]	陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰. HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(9): 402-406. doi: 10.12206/j.issn.2097-2024.202403030

期刊类型引用(0)
其他类型引用(1)

点击查看大图

计量

文章访问数: 3004
HTML全文浏览量: 286
PDF下载量: 514
被引次数: 1

正交试验优选元宝草中总黄酮的提取工艺

doi: 10.3969/j.issn.1006-0111.2015.02.011

1. 福建中医药大学药学院, 福建福州 350108;第二军医大学药学院生药学教研室, 上海 200433

2. 第二军医大学药学院生药学教研室, 上海 200433

基金项目: 上海市科委中药现代化专项 (2011DZ1970502)

收稿日期: 2013-10-17
修回日期: 2014-03-17

关键词:

元宝草 /

总黄酮 /

提取工艺 /

正交试验

摘要: 目的优选元宝草中总黄酮的提取工艺。方法在单因素试验的基础上,考察乙醇浓度、料液比、提取温度等因素对元宝草中总黄酮提取率的影响,并采用L₉(3)⁴正交试验优选最佳提取条件。结果影响元宝草中总黄酮提取率的因素顺序为乙醇浓度>料液比>提取次数>提取温度。元宝草最佳提取工艺条件为70%乙醇,提取温度60 ℃,乙醇用量20倍,提取次数3次。该条件下元宝草中总黄酮平均得率为2.38%。结论本实验设计合理、测定方法可行、实验结果可靠,为大批量从元宝草中提取总黄酮提供了理论依据。

English Abstract

Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments

1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, China;Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

2. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2013-10-17
Rev Recd Date: 2014-03-17

Keywords:

Abstract: Objective To optimize the extraction technology of total falvonoids from Hypericum sampsonii. Methods On the basis of single factor test, solid-liquid ratio, ethanol concentration, extraction time, extraction temperature and extraction times on the extraction rate were studied by taking the content of total falvonoids as indexes. Orthogonal test was conducted to establish the extraction technology of total falvonoids from Hypericum sampsonii. Results The influence degree of factors on the extraction rate of total falvonoids was in the order of ethanol concentration, solid-liquid ratio, extraction times, and temperature.The optimum extraction condition was confirmed as follows: ethanol concentration of 70%, extraction temperature of 60 ℃, and extraction times of 3×1.5 h, solid to liquid ratio of 120. Under this condition,the average extraction rate of total falvonoids reached 2.38%. Conclusion The test was reasonable in design and credible in findings, and was easily operated, which could provide scientific basis for the batch extraction of total flavonoids from Hypericum sampsonii.

龚颖珍, 郑承剑, 秦路平. 正交试验优选元宝草中总黄酮的提取工艺[J]. 药学实践与服务, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

引用本文:

龚颖珍, 郑承剑, 秦路平. 正交试验优选元宝草中总黄酮的提取工艺[J]. 药学实践与服务, 2015, 33(2): 134-137. doi: 10.3969/j.issn.1006-0111.2015.02.011

Citation:

全文HTML

参考文献 (11)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: yxsjzzs@163.com

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

正交试验优选元宝草中总黄酮的提取工艺

doi: 10.3969/j.issn.1006-0111.2015.02.011

Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments

期刊类型引用(0)

其他类型引用(1)

计量

出版历程

正交试验优选元宝草中总黄酮的提取工艺

doi: 10.3969/j.issn.1006-0111.2015.02.011

1. 福建中医药大学药学院, 福建 福州 350108;第二军医大学药学院生药学教研室, 上海 200433 2. 第二军医大学药学院生药学教研室, 上海 200433

English Abstract

Extraction process on total falvonoids from Hypericum sampsonii by orthogonal experiments

全文HTML

目录

全文HTML

1. 福建中医药大学药学院, 福建福州 350108;第二军医大学药学院生药学教研室, 上海 200433

2. 第二军医大学药学院生药学教研室, 上海 200433