Studies on content determination and extraction method of iridoid glycosides in Morinda of ficinalis How
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摘要: 目的 建立测定巴戟天环烯醚萜苷类成分含量的方法，并研究其最佳提取方法。 方法 以巴戟天环烯醚萜苷类成分水晶兰苷、去乙酰基车叶草苷酸、车叶草苷酸和车叶草苷为对照品，采用HPLC法进行含量测定。色谱柱为艾杰尔Venusil MP Cl8柱（250 mm ×4.6 mm， 5 μm），流动相为乙腈（A）-0.2%磷酸+0.01 mol/L磷酸氢二钠缓冲盐（B）梯度洗脱（0~12 min，1%~2% A；12~30 min，2%~25% A），检测波长为235 nm，流速为1.0 ml/min，柱温为25 ℃，进样量为20 μl。采用单因素考察和正交试验优化巴戟天环烯醚萜苷类成分的提取方法。 结果 水晶兰苷、去乙酰车叶草苷酸、车叶草苷酸和车叶草苷在0.375~12、0.13~4.16、0.016~0.516和0.012~0.384 μg范围内，线性关系良好；重复性、精密度、加样回收率和稳定性试验结果均符合要求。优化的巴戟天环烯醚萜苷类成分的最佳提取方法为：巴戟天药材用16倍10%乙醇浸泡9 h，渗漉提取，流速为0.8 BV/h。 结论 此法可准确、灵敏地测定巴戟天环烯醚萜苷类成分的含量，并可使该类成分得到充分提取。Abstract: Objective To develop a method for determination of iridoid glycosides in Morinda of ficinalis How. and optimize the extraction methods for iridoid glycosides in Morinda of ficinalis How. Methods The iridoid glycosides, including monotropein, deacetyl asperulosidic acid,asperulosidic acid and asperuloside as standards, HPLC method was developed to determine the content of iridoid glycosides in Morinda of ficinalis How. The separation was performed on Venusil MP C18 (250 mm×4.6 mm, 5 μm) column. The mobile phase was acetonitrile (A)-0.2% phosphoric acid and 0.01 disodium hydrogen phosphate buffer salt (B) with gradient elution (0-12 min, 1%-2% A; 12-30 min, 2%-25% A). The detection wavelength was 235 nm. The flow rate was set at 1.0 ml/min and the column temperature at 25 ℃. The injection volume was 20 μl. Single factor analysis and orthogonal test were used to optimize extraction method of iridoid glycosides in Morinda of ficinalis How. Results Monotropein, deacetyl asperulosidic acid, asperulosidic acid and asperuloside showed good linearity (r>0.999 5) in the ranges of 0.375-12 μg, 0.13-4.16 μg, 0.016-0.516 μg and 0.012-0.384 μg, respectively. This validated method has good repeatability, precision, recovery and stability. It was conformed to meet the requirements and regulation. The optimal extraction method included soaking the raw materials with 16 times of 10% ethanol for 9 h, and then extraction by percolation with the flow rate of 0.8 BV/h. Conclusion The HPLC method sensitively and precisely determined the content of iridoid glycosides in Morinda of ficinalis How. The optimized extraction method extracted these constituents effectively.
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Key words:
- Morinda of ficinalis How. /
- iridoid glycoside /
- content determination /
- HPLC /
- orthogonal test /
- percolate /
- extraction method
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[1] 国家药典委员会.中华人民共和国药典2015年版一部[S].北京:中国医药科技出版社,2015:75-76. [2] 林美珍,郑 松,田惠桥.巴戟天研究现状与展望(综述) [J].亚热带植物科学,2010,39(4):74-78. [3] 史 辑,刘梓晗,王 玲,等.HPLC测定不同产地巴戟天中5种茜草素型蒽醌的含量[J].中药材,2015,38(2):245-248. [4] 陈 娥,周 灿,廖 莎,等.不同炮制去心法对巴戟天耐斯糖含量的影响[J].湖南中医药大学学报,2016,36(4):31-33. [5] 鲍蕾蕾,吴岩斌,秦路平,等.RP-HPLC 法同时测定巴戟天中4种蒽醌类化合物的含量[J].中国药房,2010,21(15):1415-1417. [6] 章润菁,李 倩,屈敏红,等.不同产地、生长年限和种质的巴戟天药材寡糖含量分析[J].中国药学杂志,2016,51(4):315-320. [7] 李咏华,黄 裕,杨中铎,等.高效液相色谱法测定巴戟天中低聚糖含量[J].药物分析杂志,2007,27(11):1797-1799. [8] Song B,Wang JF, Wang W, et al. Effect of aqueous extract from Morinda officinalis How. on microwave-induced hypothalamic-pituitary-testis axis impairment in male sprague-dawley rats[J]. Evid-Bas Complem Altern Med, 2015,2015: 1-10. [9] Anitha T, Mohandass S. Aniti-oxidant activity of Morinda citrifolia on lymphoma bearing mice[J]. Anc Sci Life, 2006,26(1-2): 85-88. [10] Liu G, Bode A, Ma WY, et al. Two novel glycosides from the fruits of Morinda citrifolia (noni) inhibit AP-1 transactivation and cell transformation in the mouse epidermal JB6 cell line [J]. Cancer Res, 2001, 61(15): 5749-5756. [11] Shin JS, Yun KJ,Chung KS, et al. Monotropein isolated from the roots of Morinda officinalis ameliorates proinflammatory mediators in RAW 264.7 macrophages and dextran sulfate sodium (DSS)-induced colitis via NF-κB inactivation[J]. FoodChem Toxicol, 2013, 53: 263-271. [12] Kamiya K, Hamabe W, Harada S,et al. Chemical constituents of Morinda citrifolia roots exhibit hypoglycemic effects in streptozotocin-induced diabetic mice [J]. Biol Pharm Bull, 2008, 31(5): 935-938. [13] 王玉磊,崔翰明,黄世敬,等.HPLC测定不同产地和批次巴戟天中主要环烯醚萜苷含量[J].中药材,2011,34(8):1187-1190. [14] 范建伟,李艳芳,闫光军,等.HPLC同时测定茵栀黄颗粒中4个环烯醚萜苷类成分[J].中国实验方剂学杂志,2013,19(20):46-49. [15] 马养民,汪 洋.植物环烯醚萜类化合物生物活性研究进展[J].中国实验方剂学杂志,2010,16(17):234-238. [16] 刘 瑾,徐吉银,罗进辉,等.不同产地巴戟天中水晶兰苷的含量测定[J].中成药,2010,32(3):517-519. [17] 孟玲玉.中草药巴戟天有效成分提取方法的研究[J].中国科技博览,2015, 12(1):327-327. [18] 周 波,赵 臻.中草药巴戟天有效成分提取方法的研究进展[J].辽宁化工,2013,42(2): 171-173. -
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