反相高效液相色谱法测定对乙酰氨基酚血药浓度
Determination of paracetamol in human serum by HPLC
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摘要: 建立了反相高效液相色谱法测定对乙酰氨基酚血浆中浓度的方法。方法使用YWGC18柱(4.6×250mm,10μm);流动相为甲醇:醋酸-醋酸钠:二乙胺(20:80:0.5);以茶碱为内标物;血清处理用甲醇沉淀蛋白;紫外检测波长244nm;流动相流速1.5ml/min;最低检出浓度0.05μg/ml。对乙酰氨基酚血药浓度在0.25~25μg/ml范围内线性关系良好(r=0.9999)。本方法适用于对乙酸氨基酚的血药浓度测定。Abstract: An assay for paracetamol in human serum was developed using reverse phase high-performance liquid chromatographic with UV detection at 244nm. Analytial column of YWG C18 is used. The mobile phase consisted of a mixture of methanol: acetic acid-sodium acetate buffer solution: diethylamine (20:80:0.5)at the flow rate of 1.5ml/min. Theophylline is used as internal standard. Methanol is used for the sedimentation of protein. And minimum detective concentaion 0.05μg/ml. The standard curve is linear in the rang of 0.25~25μg/ml,the correlation coefficient is 0.9999.The method is suitable for the determination of human serum paracetamol and pharmacokinetic reserches.
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Key words:
- paracetamol /
- HPLC /
- plasma concentration
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